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非靶向代谢组学和伪靶向脂质组学联合肠道微生物揭示了（Thunb.）Raf. 在溃疡性结肠炎小鼠中的保护作用。

Non-targeted metabolomics and pseudo-targeted lipidomics combined with gut microbes reveal the protective effects of (Thunb.) Raf. in ulcerative colitis mice.

机构信息

Department of Anorectal Surgery, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu, China.

Department of Rheumatism and Immunity, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

出版信息

Front Cell Infect Microbiol. 2024 Oct 15;14:1397735. doi: 10.3389/fcimb.2024.1397735. eCollection 2024.

DOI:10.3389/fcimb.2024.1397735
PMID:39473926
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11518848/
Abstract

Ulcerative colitis (UC) is an inflammatory bowel disease characterized by recurrent inflammatory tissue damage to the intestinal mucosa and forming intestinal epithelial ulcers. It is one of the most intractable diseases in the world. To date, the mechanism is unclear. (Thunb.) Raf. (Wu Lianmei in Chinese; WLM), a traditional Chinese medicine, which has a long history as an anti-inflammatory, but its effect on UC was unconfirmed yet. Therefore, we established a dextran sodium sulfate (DSS)-induced UC mice model and evaluated the therapeutic effect of WLM extract. The results indicated that WLM inhibits DSS-induced inflammatory response in colitis , decrease DSS-induced clinical manifestations, reverses colon length shortening, and reduces tissue damage. The results of ELISA kits suggested that WLM could reverse the levels of DSS-induced inflammatory factors. To explore the mechanism of WLM in treating DSS-induced UC, H NMR and UHPLC-Q/Orbitrap MS were used to perform non-targeted metabolomics analysis; 21 differential metabolites in colon tissues were closely related to UC. Meanwhile, the pseudo-targeted lipidomics based on UHPLC-Q/Trap MS was used to analyze lipid metabolism disorders, and 60 differential lipid compounds were screened. These differential compounds were mainly involved in glycerophospholipid, arachidonic acid, glycerolipid, citric acid, tyrosine, and ether lipid metabolisms. The analysis of gut microbial showed that WLM may improve the symptoms of UC mice by reducing the abundance of and and increasing the abundance of and . Moreover, the real-time qPCR results showed that WLM extract could decrease the mRNA levels of inflammatory factors and may be associated with protecting the integrity of intestinal mucosal barrier by destroying metabolic pathways, especially by regulating energy and lipid metabolisms and reducing inflammatory reactions. It provides a beneficial reference for studying WLM to elucidate the therapeutic mechanism of UC.

摘要

溃疡性结肠炎（UC）是一种炎症性肠病，其特征是肠道黏膜反复发生炎症性组织损伤，并形成肠道上皮溃疡。它是世界上最难治愈的疾病之一。迄今为止，其发病机制尚不清楚。（Thunb.） Raf.（吴莲梅，中文名称；WLM），一种中药，具有悠久的抗炎作用，但它对 UC 的作用尚未得到证实。因此，我们建立了葡聚糖硫酸钠（DSS）诱导的 UC 小鼠模型，并评估了 WLM 提取物的治疗效果。结果表明，WLM 抑制结肠炎中 DSS 诱导的炎症反应，降低 DSS 诱导的临床表现，逆转结肠缩短，并减轻组织损伤。ELISA 试剂盒的结果表明，WLM 可以逆转 DSS 诱导的炎症因子水平。为了探讨 WLM 治疗 DSS 诱导的 UC 的机制，采用 1H NMR 和 UHPLC-Q/Orbitrap MS 进行非靶向代谢组学分析；在结肠组织中鉴定出 21 个与 UC 密切相关的差异代谢物。同时，基于 UHPLC-Q/Trap MS 的拟靶向脂质组学用于分析脂质代谢紊乱，筛选出 60 个差异脂质化合物。这些差异化合物主要涉及甘油磷脂、花生四烯酸、甘油酯、柠檬酸、酪氨酸和醚脂代谢。肠道微生物分析表明，WLM 可能通过降低和的丰度和增加和的丰度来改善 UC 小鼠的症状。此外，实时 qPCR 结果表明，WLM 提取物可以降低炎症因子的 mRNA 水平，这可能与通过破坏代谢途径保护肠道黏膜屏障的完整性有关，特别是通过调节能量和脂质代谢以及减轻炎症反应。这为研究 WLM 以阐明 UC 的治疗机制提供了有益的参考。

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Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.芹菜素重塑肠道微生物群以改善溃疡性结肠炎。
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Protective effect of HFY06 on dextran sulfate sodium-induced colitis in mice.
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Pulsatilla decoction improves DSS-induced colitis via modulation of fecal-bacteria-related short-chain fatty acids and intestinal barrier integrity.白头翁汤通过调节粪便细菌相关的短链脂肪酸和肠道屏障完整性改善 DSS 诱导的结肠炎。
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