Sarkar Jyotirmay, Singh Rajveer, Chandel Shivani
Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab, India.
Proteomics Clin Appl. 2025 Jan;19(1):e202400048. doi: 10.1002/prca.202400048. Epub 2024 Oct 30.
Liquid chromatography, when used in conjunction with mass spectrometry (LC/MS), is a powerful tool for conducting accurate and reproducible investigations of numerous metabolites in natural products (NPs). LC/MS has gained prominence in metabolomic research due to its high throughput, the availability of multiple ionization techniques and its ability to provide comprehensive metabolite coverage. This unique method can significantly influence various scientific domains. This review offers a comprehensive overview of the current state of LC/MS-based metabolomics in the investigation of NPs. This review provides a thorough overview of the state of the art in LC/MS-based metabolomics for the investigation of NPs. It covers the principles of LC/MS, various aspects of LC/MS-based metabolomics such as sample preparation, LC modes, method development, ionization techniques and data pre-processing. Moreover, it presents the applications of LC/MS-based metabolomics in numerous fields of NPs research such as including biomarker discovery, the agricultural research, food analysis, the study of marine NPs and microbiological research. Additionally, this review discusses the challenges and limitations of LC/MS-based metabolomics, as well as emerging trends and developments in this field.
液相色谱与质谱联用（LC/MS）时，是对天然产物（NP）中的多种代谢物进行准确且可重复研究的强大工具。由于其高通量、多种电离技术的可用性以及提供全面代谢物覆盖范围的能力，LC/MS在代谢组学研究中已占据突出地位。这种独特的方法可对多个科学领域产生重大影响。本综述全面概述了基于LC/MS的代谢组学在天然产物研究中的现状。本综述全面概述了基于LC/MS的代谢组学用于天然产物研究的最新技术水平。它涵盖了LC/MS的原理、基于LC/MS的代谢组学的各个方面，如样品制备、液相色谱模式、方法开发、电离技术和数据预处理。此外，它还介绍了基于LC/MS的代谢组学在天然产物研究的众多领域中的应用，包括生物标志物发现、农业研究、食品分析、海洋天然产物研究和微生物研究。此外，本综述讨论了基于LC/MS的代谢组学的挑战和局限性，以及该领域的新兴趋势和发展。
