Department of Rheumatology and Immunology, The First Affiliated Hospital of Wannan Medical College, Wuhu, China.
Clin Rheumatol. 2024 Dec;43(12):3993-3998. doi: 10.1007/s10067-024-07209-7. Epub 2024 Oct 30.
To investigate the significance of CXC chemokine ligand 10 (CXCL10) in the pathogenesis of isolated polymyalgia rheumatica (PMR).
The serum of six PMR patients diagnosed and treated at the First Affiliated Hospital of Wannan Medical College from September 2019 to December 2020 before treatment and after remission was collected, and the serum of six active rheumatoid arthritis (RA) patients and six healthy medical checkups were also collected, and protein microarray technology was used to detect 24 cytokines, including IL-6, IL-4, CXCL10, CXCL8, and CXCL2. Subsequently, serum was collected from other 28 patients with active PMR, 26 patients with PMR in remission, 24 patients with active RA, and 24 healthy medical checkups who were diagnosed and treated at the First Affiliated Hospital of Wannan Medical College from January 2021 to July 2023, and the enzyme-linked immunosorbent assay (ELISA) was used to validate and compare the levels of CXCL10 in each group and analyze the correlation between the levels of serum CXCL10 and the parameters of the clinical activities of PMR.
Protein microarray screening revealed significant differences in CXCL10 before and after PMR treatment, and ELISA validation revealed that peripheral serum CXCL10 levels were significantly higher in the PMR-active group than in the remission group (P < 0.001), and also significantly higher than in the RA-active group (P = 0.003) and in the healthy control group (P < 0.001); correlation analysis showed a significant positive correlation between serum CXCL10 levels and serum ferritin in PMR patients (r = 0.450, P = 0.024). In the ROC curve for distinguishing PMR and RA, the area under the curve is 0.741, sensitivity = 0.643, and specificity = 0.792.
CXCL10 may play a role in the pathogenesis of isolated PMR and its level might contribute to the differential diagnosis of PMR and RA. Key Points • The concentration of CXCL10 was higher in peripheral blood of isolated PMR patients. • CXCL10 is a potential diagnostic biomarker for isolated PMR patients. • The level of CXCL10 might contribute to the differential diagnosis of PMR and RA.
探讨趋化因子配体 10（CXCL10）在孤立性巨细胞动脉炎（PMR）发病机制中的意义。
收集 2019 年 9 月至 2020 年 12 月皖南医学院第一附属医院确诊并治疗的 6 例 PMR 患者治疗前后的血清，同时收集 6 例活动性类风湿关节炎（RA）患者和 6 例健康体检者的血清，采用蛋白质微阵列技术检测 24 种细胞因子，包括 IL-6、IL-4、CXCL10、CXCL8 和 CXCL2。随后，收集 2021 年 1 月至 2023 年 7 月在皖南医学院第一附属医院确诊并治疗的 28 例活动性 PMR 患者、26 例 PMR 缓解患者、24 例活动性 RA 患者和 24 例健康体检者的血清，采用酶联免疫吸附试验（ELISA）验证和比较各组 CXCL10 水平，并分析血清 CXCL10 水平与 PMR 临床活动参数的相关性。
蛋白质微阵列筛选发现 PMR 治疗前后 CXCL10 有显著差异，ELISA 验证发现 PMR 活动组外周血清 CXCL10 水平明显高于缓解组（P<0.001），也明显高于 RA 活动组（P=0.003）和健康对照组（P<0.001）；相关性分析显示 PMR 患者血清 CXCL10 水平与血清铁蛋白呈显著正相关（r=0.450，P=0.024）。在 PMR 和 RA 的鉴别诊断 ROC 曲线中，曲线下面积为 0.741，灵敏度为 0.643，特异性为 0.792。
CXCL10 可能在孤立性 PMR 的发病机制中起作用，其水平可能有助于 PMR 和 RA 的鉴别诊断。
关键点
•孤立性 PMR 患者外周血 CXCL10 浓度较高。
•CXCL10 可能是孤立性 PMR 患者的潜在诊断生物标志物。
•CXCL10 水平可能有助于 PMR 和 RA 的鉴别诊断。
