Jiangxi Province Key Laboratory of Immunology and Inflammation, Jiangxi Provincial Clinical Research Center for Laboratory Medicine, Department of Clinical Laboratory, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Mingde Road No.1, Nanchang, 330006, Jiangxi, People's Republic of China.
School of Public Health, Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Sci Rep. 2024 Oct 30;14(1):26122. doi: 10.1038/s41598-024-77746-3.
This research aimed to analyze the volatile compounds emitted during the proliferation of Klebsiella pneumoniae (K. pneumoniae) in the laboratory setting using gas chromatography-ion mobility spectrometry (GC-IMS) and to investigate the potential of volatile metabolomics for detecting carbapenemase-producing strains of K. pneumoniae. The volatile metabolomics of K. pneumoniae were comprehensively analyzed using GC-IMS in tryptic soy broth (TSB) as the culture medium. Afterward, the growth stabilization period (T2) served as the primary time point for analysis, with the introduction of imipenem and carbapenemase inhibitors (avibactam sodium or EDTA) during the exponential growth phase (T0) to further investigate alterations in volatile molecules associated with K. pneumoniae. Standard strains were utilized as references, while clinical strains were employed for validation purposes. At T2, a total of 22 volatile organic compounds (VOCs) associated with K. pneumoniae were identified (3 VOCs found in both monomer and dimer forms). Significant differences in VOCs were observed between carbapenemase-negative and carbapenemase-positive strains, both standard and clinical, following the introduction of imipenem. Furthermore, the addition of avibactam sodium led to distinct changes in the VOC content of strains producing class A carbapenemase, while the addition of EDTA resulted in specific alterations in the volatile metabolic profiles of strains producing class B carbapenemase. GC-IMS demonstrated significant promise for analyzing bacterial volatile metabolomics, and its application in evaluating the volatolomics of K. pneumoniae may facilitate the timely detection of carbapenemase-producing strains.
本研究旨在使用气相色谱-离子迁移谱（GC-IMS）分析实验室条件下肺炎克雷伯菌（K. pneumoniae）增殖过程中释放的挥发性化合物，并探讨挥发性代谢组学在检测产碳青霉烯酶肺炎克雷伯菌中的潜在应用。在胰蛋白酶大豆肉汤（TSB）培养基中，全面分析肺炎克雷伯菌的挥发性代谢组学。随后，在生长稳定期（T2）作为主要分析时间点，在指数生长期（T0）引入亚胺培南和碳青霉烯酶抑制剂（阿维巴坦钠或 EDTA），进一步研究与肺炎克雷伯菌相关的挥发性分子变化。使用标准菌株作为参考，同时使用临床菌株进行验证。在 T2 时，鉴定出与肺炎克雷伯菌相关的 22 种挥发性有机化合物（3 种在单体和二聚体形式下都存在的 VOCs）。在引入亚胺培南后，无论是标准菌株还是临床菌株，产碳青霉烯酶阴性和阳性菌株之间的 VOCs 均存在显著差异。此外，添加阿维巴坦钠导致产 A 型碳青霉烯酶的菌株的 VOC 含量发生明显变化，而添加 EDTA 则导致产 B 型碳青霉烯酶的菌株的挥发性代谢图谱发生特定变化。GC-IMS 显示出分析细菌挥发性代谢组学的巨大潜力，其在评估肺炎克雷伯菌的挥发组学方面的应用可能有助于及时检测产碳青霉烯酶的菌株。
