Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, China.
National Key Laboratory of Meat Quality Control and Cultured Meat Development, Nanjing, 210095, China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae338.
The aim of this study was to investigate the alleviating effects and mechanisms of bile acids (BA) on corticosterone-induced fatty liver in broiler chickens. Male Arbor Acres chickens were randomly divided into 3 groups: control group (CON), stress model group (CORT), and BA-treated group (CORT-BA). The CORT-BA group received a diet with 250 mg/kg BA from 21 d of age. From days 36 to 43, both the CORT and CORT-BA groups received subcutaneous injections of corticosterone to simulate chronic stress. The results indicated that BA significantly mitigated the body weight loss, liver enlargement, and hepatic lipid deposition caused by corticosterone (P < 0.05). Liver RNA-seq analysis showed that BA alleviated corticosterone-induced fatty liver by inhibiting lipid metabolism pathways, including fatty acid biosynthesis, triglyceride biosynthesis, and fatty acid transport. Additionally, BA improved corticosterone-induced downregulation of glucocorticoid receptor (GR) expression (P < 0.05). Molecular docking and cellular thermal shift assays revealed that hyodeoxycholic acid (HDCA), a major component of compound BA, could bind to GR and enhance its stability. In conclusion, BA alleviated corticosterone-induced fatty liver in broilers by inhibiting lipid synthesis pathways and mitigating the suppression of hepatic GR expression.
本研究旨在探讨胆汁酸（BA）对皮质酮诱导肉鸡脂肪肝的缓解作用及其机制。雄性爱拔益加（Arbor Acres）鸡随机分为 3 组：对照组（CON）、应激模型组（CORT）和 BA 处理组（CORT-BA）。CORT-BA 组从 21 日龄开始摄入含有 250mg/kg BA 的饮食。从第 36 天到第 43 天，CORT 和 CORT-BA 组均接受皮下注射皮质酮以模拟慢性应激。结果表明，BA 显著减轻了皮质酮引起的体重减轻、肝脏肿大和肝脂质沉积（P<0.05）。肝 RNA-seq 分析表明，BA 通过抑制脂质代谢途径，包括脂肪酸合成、甘油三酯合成和脂肪酸转运，缓解了皮质酮诱导的脂肪肝。此外，BA 改善了皮质酮诱导的糖皮质激素受体（GR）表达下调（P<0.05）。分子对接和细胞热转移分析表明，熊去氧胆酸（HDCA）是 BA 的主要成分之一，可与 GR 结合并增强其稳定性。综上所述，BA 通过抑制脂质合成途径和减轻肝 GR 表达抑制，缓解了皮质酮诱导的肉鸡脂肪肝。
