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中国流行 ST37 型菌株的基因组和表型特征分析。

Genomic and phenotypic characterization of a strain of the epidemic ST37 type from China.

机构信息

Department of Infection Control Center of Xiangya Hospital, Central South University, Changsha, China.

Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, United States.

出版信息

Front Cell Infect Microbiol. 2024 Oct 18;14:1412408. doi: 10.3389/fcimb.2024.1412408. eCollection 2024.

DOI:10.3389/fcimb.2024.1412408
PMID:39492989
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11527712/
Abstract

strains of sequence type (ST) 37, primarily including PCR ribotype (RT) 017, are prevalent in mainland China. Our study aimed to compare the major virulence factors of an epidemic isolate of ST37 type (Xy06) from China with the well-characterized reference strains R20291 (RT027) and CD630E (ST54), as well as a Chinese ST54 strain (Xy07) isolated from the same hospital. The Xy06 genome was predicted to harbor two complete prophages and several transposon-like elements. Comparative analysis of PaLoc revealed a truncated tcdA gene, a functional tcdB gene, a functional tcdC gene, and well-conserved tcdR and tcdE genes. Phenotypic comparisons showed that Xy06 was a robust producer of TcdB, readily sporulated and germinated, and strongly bound to human gut epithelial cells. In a mouse model of infection, Xy06 was more virulent than strains CD630E and Xy07 and was comparable to strain R20291 in virulence. Our data suggest the potential threat of the epidemic ST37 strains in China.

摘要

序列型 (ST) 37 株，主要包括 PCR 核糖型 (RT) 017，在中国流行。本研究旨在比较来自中国的流行 ST37 型 (Xy06) 分离株与特征明确的参考株 R20291 (RT027) 和 CD630E (ST54) 以及同一医院分离的中国 ST54 株 (Xy07) 的主要毒力因子。Xy06 基因组预测含有两个完整的前噬菌体和几个转座子样元件。PaLoc 的比较分析显示截短的 tcdA 基因、功能的 tcdB 基因、功能的 tcdC 基因以及保守的 tcdR 和 tcdE 基因。表型比较显示 Xy06 是 TcdB 的强产生菌，易形成孢子和发芽，并与人肠道上皮细胞强烈结合。在感染小鼠模型中，Xy06 的毒力比 CD630E 和 Xy07 株更强，与 R20291 株的毒力相当。我们的数据表明中国流行的 ST37 株具有潜在威胁。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/eb89b3bc8254/fcimb-14-1412408-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/8cdf1663bf0c/fcimb-14-1412408-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/793aa78dc0e3/fcimb-14-1412408-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/bd80c2165581/fcimb-14-1412408-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/692bb5360e8e/fcimb-14-1412408-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/82e2f0de3e6e/fcimb-14-1412408-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/8c8f9e6e01e1/fcimb-14-1412408-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/eb89b3bc8254/fcimb-14-1412408-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/8cdf1663bf0c/fcimb-14-1412408-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/793aa78dc0e3/fcimb-14-1412408-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/bd80c2165581/fcimb-14-1412408-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/692bb5360e8e/fcimb-14-1412408-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/82e2f0de3e6e/fcimb-14-1412408-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/8c8f9e6e01e1/fcimb-14-1412408-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8251/11527712/eb89b3bc8254/fcimb-14-1412408-g007.jpg

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[1]
Genomic and phenotypic characterization of a strain of the epidemic ST37 type from China.

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[2]
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[3]
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[9]
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[10]
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本文引用的文献

[1]
Global evolutionary dynamics and resistome analysis of ribotype 017.

Microb Genom. 2022-3

[2]
A narrative review of Clostridioides difficile infection in China.

Anaerobe. 2022-4

[3]
Complete Genome Sequencing and Comparative Phenotypic Analysis Reveal the Discrepancy Between ST81 and ST37 Isolates.

Front Microbiol. 2021-12-21

[4]
Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.

Nat Commun. 2021-2-18

[5]
Clostridioides difficile phage biology and application.

FEMS Microbiol Rev. 2021-9-8

[6]
Functional analyses of epidemic Clostridioides difficile toxin B variants reveal their divergence in utilizing receptors and inducing pathology.

PLoS Pathog. 2021-1

[7]
Molecular Epidemiology and Risk Factors of ST81 Infection in a Teaching Hospital in Eastern China.

Front Cell Infect Microbiol. 2020

[8]
Protein Sequence Analysis Using the MPI Bioinformatics Toolkit.

Curr Protoc Bioinformatics. 2020-12

[9]
Subtyping analysis reveals new variants and accelerated evolution of Clostridioides difficile toxin B.

Commun Biol. 2020-7-3

[10]
Isolation and Characterization of the Novel Phage JD032 and Global Transcriptomic Response during JD032 Infection of Clostridioides difficile Ribotype 078.

mSystems. 2020-5-5

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