Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2025;2869:1-5. doi: 10.1007/978-1-0716-4204-7_1.
The major environmental factors limiting rice growth and production are osmotic stresses such as drought and high salinity. High osmotic stresses directly disrupt cellular activities, leading to plant growth retardation or death. Plants have various response mechanisms to survive under such stresses. Understanding rice's stress response mechanisms is necessary to enhance the osmotic stress tolerance of rice. However, assessing specific physiological responses to osmotic stresses is difficult because multiple environmental factors affect rice growth. Here, we describe a simple method for analyzing the osmotic stress responses of rice plants using a hydroponic culture system. This method allows comprehensive gene expression and phenotypic analyses under osmotic stress conditions in rice. Various osmotic stress conditions and samples can be tested simultaneously because this method is small-scale. In addition, the procedure is easy, and highly reproductive results can be obtained.
限制水稻生长和生产的主要环境因素是渗透胁迫，如干旱和高盐度。高渗透压直接破坏细胞活动，导致植物生长迟缓或死亡。植物有各种应对机制来在这种胁迫下生存。了解水稻的胁迫反应机制对于提高水稻的渗透胁迫耐受性是必要的。然而，由于多种环境因素会影响水稻的生长，因此评估其对渗透胁迫的特定生理反应是很困难的。在这里，我们描述了一种使用水培培养系统分析水稻植株渗透胁迫反应的简单方法。该方法允许在水稻的渗透胁迫条件下进行全面的基因表达和表型分析。由于该方法规模较小，因此可以同时测试各种渗透胁迫条件和样本。此外，该程序简单，可获得高度重现的结果。
