Zhang Weifeng, Li Jirui, Xie Fengshan, Zeng Liting, Hong Liangli, Li Penghao, Yan Xiaomiao, Xu Jingliang, Du Meina, Hong Jiongzhi, Yi Dingrong, Xie Jiahao, Gu Jiang
Laboratory of Molecular Pathology Department of Pathology and Pathophysiology Shantou University Medical College Shantou China.
Jinxin Research Institute for Reproductive Medicine and Genetics Xinan Hospital for Maternal and Child Health Care Chengdu China.
Eng Life Sci. 2024 Oct 13;24(11):e202400062. doi: 10.1002/elsc.202400062. eCollection 2024 Nov.
Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology and biomedical research, but there is still a need to shorten the operation process and reduce the cost of antibodies. This study aims to assess a novel IHC technique that incorporates mechanical microvibration (MMV) to expedite the process, reduce antibody consumption, and enhance staining quality. MMV was generated using coin vibration motors attached to glass slides mounted with consecutive tissue sections. Multiple antibodies targeting various antigens were used to stain cancerous and normal tissues, with and without microvibration. Various parameters were tested, including incubation durations, temperatures, and antibody dilutions. The novel method showed the potential to achieve comparable or superior outcomes in significantly less time, utilizing over 10 times less antibody than controls. MMV improved specific staining quality, yielding stronger, and better-defined positive reactions. This was validated through a multicenter double-blind assessment and quantitative image analysis. The possible mechanisms were also investigated. MMV shortens immunohistochemical staining duration, reduces antibody usage, and enhances staining specificity, likely by accelerating antibody movement and diffusion. These improvements translate to time and cost savings, offering clinical and financial value for diagnostic pathology and biomedical research.
免疫组织化学（IHC）是诊断病理学和生物医学研究中广泛使用的技术，但仍需要缩短操作过程并降低抗体成本。本研究旨在评估一种结合机械微振动（MMV）的新型免疫组织化学技术，以加快操作过程、减少抗体消耗并提高染色质量。使用附着在装有连续组织切片的载玻片上的硬币振动电机产生微振动。使用多种针对不同抗原的抗体对癌组织和正常组织进行染色，分别采用有微振动和无微振动的方式。测试了包括孵育时间、温度和抗体稀释度等各种参数。该新方法显示出在显著更短的时间内取得可比或更好结果的潜力，抗体用量比对照组少10倍以上。微振动改善了特异性染色质量，产生更强且更清晰的阳性反应。这通过多中心双盲评估和定量图像分析得到验证。还研究了可能的机制。微振动可能通过加速抗体移动和扩散来缩短免疫组织化学染色时间、减少抗体使用并提高染色特异性。这些改进带来了时间和成本的节省，为诊断病理学和生物医学研究提供了临床和经济价值。
