Siska Siska, Wiratnaya I Gede Eka, Bakta I Made, Jawi I Made, Widiana I Gde Raka, Yuliawati Putu, Saraswati Made Ratna, Suroto Heri
Department of Ophthalmology, Faculty of Medicine, Udayana University, Denpasar, Indonesia.
Department of Orthopedics and Traumatology, Faculty of Medicine, Udayana University, Denpasar, Indonesia.
Rambam Maimonides Med J. 2024 Oct 28;15(4):e0017. doi: 10.5041/RMMJ.10531.
A single layer of tightly spaced cells, known as the endothelium, rests on the posterior side of the cornea. This endothelium regulates the stroma's relative dehydration, which is essential for corneal clarity. Cell therapy is an innovative method being used to repair various corneal abnormalities. Mesenchymal stem cells (MSCs) are now one of the most significant types of stem cells scientists have studied. This study aimed to evaluate the role of MSCs for corneal endothelial regeneration.
A systematic review was performed by searching for articles from reputable databases with many study-type references, including PubMed, Cochrane Library, Science Direct, and Google Scholar, up to January 2024. The resulting data were displayed using the 2020 PRISMA flowchart and evaluated using the PRISMA 2020 checklist. Most of the included studies were in vivo and used topical application and anterior chamber injection as the administration routes.
Based on the findings of this review, MSCs increased corneal endothelial cell density, improved the defect area and corneal transparency, facilitated endothelial cell regeneration and wound healing, and decreased neovascularization and corneal pro-inflammatory cytokines as compared to controls.
Administration of MSCs into the anterior chamber could increase regeneration and proliferation of corneal endothelial tissue.
一层紧密排列的细胞，即内皮细胞，位于角膜后侧。该内皮细胞调节基质的相对脱水，这对角膜透明度至关重要。细胞疗法是一种用于修复各种角膜异常的创新方法。间充质干细胞（MSCs）现在是科学家研究的最重要的干细胞类型之一。本研究旨在评估间充质干细胞在角膜内皮再生中的作用。
通过检索截至2024年1月来自包括PubMed、Cochrane图书馆、Science Direct和谷歌学术等具有众多研究类型参考文献的知名数据库中的文章进行系统综述。所得数据使用2020年PRISMA流程图展示，并使用PRISMA 2020清单进行评估。纳入的大多数研究是在体内进行的，使用局部应用和前房注射作为给药途径。
基于本综述的结果，与对照组相比，间充质干细胞增加了角膜内皮细胞密度，改善了缺损区域和角膜透明度，促进了内皮细胞再生和伤口愈合，并减少了新生血管形成和角膜促炎细胞因子。
将间充质干细胞注入前房可增加角膜内皮组织的再生和增殖。
