Suppr超能文献

采用顶空固相微萃取-气相色谱-质谱联用和代谢组学技术鉴定红葡萄柚在红曲菌发酵过程中的特征挥发物和代谢途径。

Identification of characteristic volatiles and metabolomic pathways during the fermentation of red grapefruit by Monascus purpureus using HS-SPME-GC-MS and metabolomics.

机构信息

School of Food and Health, Beijing Technology & Business University (BTBU), Beijing 100048, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China.

School of Food and Health, Beijing Technology & Business University (BTBU), Beijing 100048, China.

出版信息

Food Chem. 2025 Feb 1;464(Pt 3):141786. doi: 10.1016/j.foodchem.2024.141786. Epub 2024 Oct 28.

Abstract

Fermentation of red grapefruit by Monascus purpureus (M. purpureus) results in complex changes in flavor compounds and metabolic profiles, but the specifics of these alterations are not well understood. This study aimed to investigate the changes in flavor compounds and metabolomic traits during this fermentation process. Using Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) with non-targeted metabolomics, we analyzed flavor compounds and measured physicochemical indices throughout the fermentation period. We identified 23 volatile flavor metabolites before and after fermentation, focusing on acids, alcohols, and aldehydes, of these, 9 showed an upward effect and 14 showed a downward effect. Key metabolic pathways involved included butyric acid, taurine, and hypotaurine, with notable downregulation of acetone and 1-butanol in the butyric acid pathway. The study reveals that butyric acid-related metabolism influences other pathways such as glycolysis, fatty acid metabolism, and the tricarboxylic acid cycle in M. purpureus. These findings provide insights into the generation of flavor compounds during fermentation and offer a theoretical basis for the industrial production of fermented citrus fruits.

摘要

红葡萄柚经红曲菌(Monascus purpureus,M. purpureus)发酵后,风味化合物和代谢物图谱会发生复杂变化,但具体变化情况尚不清楚。本研究旨在探讨该发酵过程中风味化合物和代谢组学特征的变化。采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)结合非靶向代谢组学方法,分析发酵前后的风味化合物和理化指标。我们共鉴定出 23 种挥发性风味代谢物,主要涉及酸、醇和醛类化合物,其中 9 种呈上升趋势,14 种呈下降趋势。涉及的关键代谢途径包括丁酸、牛磺酸和次牛磺酸,丁酸途径中丙酮和 1-丁醇显著下调。该研究揭示了红曲菌中丁酸相关代谢物会影响糖酵解、脂肪酸代谢和三羧酸循环等其他途径。这些发现为发酵过程中风味化合物的产生提供了深入了解,并为发酵柑橘类水果的工业化生产提供了理论依据。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验