Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, China.
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
NPJ Biofilms Microbiomes. 2024 Nov 12;10(1):124. doi: 10.1038/s41522-024-00600-x.
Environmental osmolarity plays a crucial role in regulating the functions and behaviors of both host cells and pathogens. However, it remains unclear whether and how environmental osmotic stimuli modulate bacterial‒host interfacial adhesion. Using single-cell force spectroscopy, we revealed that the interfacial adhesion force depended nonlinearly on the osmotic prestimulation of host cells but not bacteria. Quantitatively, the adhesion force increased dramatically from 25.98 nN under isotonic conditions to 112.45 or 93.10 nN after the host cells were treated with the hypotonic or hypertonic solution. There was a strong correlation between the adhesion force and the number of host cells harboring adherent/internalized bacteria. We further revealed that enhanced overexpression levels of collagen XV and II were responsible for the increases in interfacial adhesion under hypotonic and hypertonic conditions, respectively. This work provides new opportunities for developing host-directed antibacterial strategies related to interfacial adhesion from a mechanobiological perspective.
环境渗透压在调节宿主细胞和病原体的功能和行为方面起着至关重要的作用。然而，目前尚不清楚环境渗透刺激是否以及如何调节细菌-宿主界面粘附。使用单细胞力谱技术，我们揭示了界面粘附力与宿主细胞的渗透压预刺激呈非线性相关，但与细菌无关。定量分析表明，在等渗条件下，粘附力为 25.98nN，而在用低渗或高渗溶液处理宿主细胞后，粘附力分别急剧增加到 112.45 或 93.10nN。粘附力与含有粘附/内化细菌的宿主细胞数量之间存在很强的相关性。我们进一步揭示，在低渗和高渗条件下，胶原 XV 和 II 的过度表达水平增强分别导致界面粘附力的增加。这项工作从机械生物学的角度为开发与界面粘附相关的宿主定向抗菌策略提供了新的机会。
