Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France.
Sci Data. 2024 Nov 12;11(1):1214. doi: 10.1038/s41597-024-04060-y.
The cerebral cortex extends axonal projections to several subcortical brain regions, including the striatum, thalamus, superior colliculus, and pontine nuclei. Experimental tract-tracing studies have shown that these subcortical projections are topographically organized, reflecting the spatial organization of sensory surfaces and body parts. Several public collections of mouse- and rat- brain tract-tracing data are available, with the Allen mouse brain connectivity atlas being most prominent. There, a large body of image data can be inspected, but it is difficult to combine data from different experiments and compare spatial distribution patterns. To enable co-visualization and comparison of topographical organization in mouse brain cortico-subcortical projections across experiments, we represent axonal labelling data as point data in a common 3D brain atlas space. We here present a collection of point-cloud data representing spatial distribution of corticostriatal, corticothalamic, corticotectal, and corticopontine projections in mice and exemplify how these spatially integrated point data can be used as references for experimental investigations of topographic organization in transgenic mice, and for cross-species comparison with corticopontine projections in rats.
大脑皮层向包括纹状体、丘脑、上丘和脑桥核在内的几个皮质下脑区延伸轴突投射。实验示踪研究表明，这些皮质下投射具有拓扑组织，反映了感觉表面和身体部位的空间组织。有几个公开发布的小鼠和大鼠脑追踪数据集合，其中 Allen 小鼠脑连接图谱最为突出。在那里，可以检查大量的图像数据，但很难将来自不同实验的数据组合并比较空间分布模式。为了能够在实验之间共同可视化和比较小鼠大脑皮质下投射的拓扑组织，我们将轴突标记数据表示为常见的 3D 大脑图谱空间中的点数据。我们在此展示了一个点云数据集，代表了小鼠皮质纹状体、皮质丘脑、皮质顶盖和皮质脑桥投射的空间分布，并举例说明了如何将这些空间集成的点数据用作转基因小鼠中拓扑组织实验研究的参考，以及与大鼠皮质脑桥投射的跨物种比较。
