Cellulase immobilization on nano-chitosan/chromium metal-organic framework hybrid matrix for efficient conversion of lignocellulosic biomass to glucose.

In the current work, cellulase from was successfully immobilized on a novel epoxy-affixed chromium metal-organic framework/chitosan (Cr@-MIL-101/CS) support via covalent method using glutaraldehyde as a crosslinker. The bare and cellulase-bound support was characterized by using various microscopic and spectroscopic techniques. Immobilized cellulase exhibited a high immobilization yield of 0.7 ± 0.01 mg/cm, retaining 87.5 ± 0.04% of its specific activity and displaying enhanced catalytic performance. The immobilized enzyme was maximally active at pH 5.0, temperature 65 °C and 0.9 × 10-2 mg/ml saturating substrate concentration and the half-lives of free and immobilized cellulases were approximately 9 and 19 days, respectively. The decrease in activation energy, enthalpy change, and Gibbs free energy change, coupled with an increase in entropy change upon immobilization, indicated that the enzyme's efficiency, stability, and spontaneity in catalyzing the reaction were enhanced by immobilization. Additionally, the immobilized cellulase efficiently converted rice husk cellulose to glucose, with a quantification limit of 0.05%, linear measurement ranging from 0.1 to 0.9%, and 8.5% conversion efficiency. The present method exhibited a strong correlation (R = 0.998) with the DNS method, validating its reliability. Notably, the epoxy/Cr@-MIL-101/CS-bound cellulase demonstrated impressive thermal and pH stabilities, retaining 50% of its activity at 75 °C and over 96% at pH levels of 4.5 and 5.0 after 12 h. Furthermore, it showed excellent reusability, preserving 80% of its activity after 15 cycles and maintaining 50% of its activity even after 20 days of storage. These results suggest that epoxy/Cr@-MIL-101/CS/cellulase composites could be very effective for large-scale cellulose hydrolysis applications.