一种对铜离子具有高亲和力和选择性的 DNA 适体。
A high affinity and selective DNA aptamer for copper ions.
机构信息
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
出版信息
Chem Commun (Camb). 2024 Nov 28;60(96):14272-14275. doi: 10.1039/d4cc05410e.
Capture-SELEX was employed for the selection of DNA aptamers for Cu. The best aptamer named Cu-1 has a of 14.2 nM as determined using the strand-displacement assay, representing an approximate 3000-fold improvement over a previously reported Cu aptamer. The sensor achieved a limit of detection of 2.1 nM.
采用捕获式 SELEX 技术对铜离子（Cu ）进行了 DNA 适体的筛选。通过链置换分析法测定，筛选得到的最佳适体 Cu-1 的解离常数（Kd ）为 14.2 nM，与之前报道的铜离子适体相比，提高了约 3000 倍。该传感器的检测限达到 2.1 nM。
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