用曲拉通X-100溶解人红细胞膜糖蛋白
Solubilization of human erythrocyte membrane glycoproteins by triton X-100.
作者信息
Pratt R S, Cook G M
出版信息
Biochem J. 1979 May 1;179(2):299-303. doi: 10.1042/bj1790299.
Abstract
- The enzymic removal of sialic acid residues from the glycoproteins of the human erythrocyte decreases the solubilization of membrane glycoprotein by Triton X-100. 2. The solubilization of asialoglycoprotein by Triton X-100 may be restored by the addition of borate. 3. Use of this non-ionic detergent in the presence of borate, as a general procedure for the mild solubilization of membrane glycoproteins deficient in sialic acid residues, is discussed.
摘要
- 从人红细胞糖蛋白上酶促去除唾液酸残基会降低Triton X-100对膜糖蛋白的增溶作用。2. 添加硼酸盐可恢复Triton X-100对去唾液酸糖蛋白的增溶作用。3. 讨论了在硼酸盐存在下使用这种非离子型去污剂作为温和增溶缺乏唾液酸残基的膜糖蛋白的通用方法。
相似文献
1
Solubilization of human erythrocyte membrane glycoproteins by triton X-100.用曲拉通X-100溶解人红细胞膜糖蛋白
Biochem J. 1979 May 1;179(2):299-303. doi: 10.1042/bj1790299.
2
The band 3-rich membrane of llama erythrocytes: studies on cell shape and the organization of membrane proteins.羊驼红细胞富含带3蛋白的膜：关于细胞形状和膜蛋白组织的研究
J Membr Biol. 1983;72(3):161-71. doi: 10.1007/BF01870583.
3
The relationship of detergent-solubilization plasma-membrane components of rabbit alveolar macrophages to an isolated inhibitor of phagocytosis.兔肺泡巨噬细胞的去污剂增溶质膜成分与一种分离出的吞噬作用抑制剂之间的关系。
Biochem J. 1979 May 1;179(2):305-14. doi: 10.1042/bj1790305.
4
Rapid quantitative separation of the major glycoproteins (PAS 1, 2, and 3) from other human red cell membrane proteins in a nondenaturing medium by affinity chromatography.
Anal Biochem. 1977 Jun;80(2):438-45. doi: 10.1016/0003-2697(77)90666-2.
5
A carbohydrate-deficient membrane glycoprotein in human erythrocytes of phenotype S-s-.S-s-表型人红细胞中一种缺乏碳水化合物的膜糖蛋白。
Biochem J. 1977 Jul 1;165(1):157-61. doi: 10.1042/bj1650157.
6
Selective spin labeling of sialic acid residues of glycoproteins and glycolipids in erythrocyte membranes: a novel method to study cell surface interactions.
FEBS Lett. 1980 Jun 30;115(2):185-8. doi: 10.1016/0014-5793(80)81164-1.
7
Integral membrane protein interaction with Triton cytoskeletons of erythrocytes.
Biochim Biophys Acta. 1979 Oct 19;557(1):122-34. doi: 10.1016/0005-2736(79)90095-6.
8
The degradation of band-3 protein in Triton X-100 extracts of the human erythrocyte membrane [proceedings].
Biochem Soc Trans. 1980 Jun;8(3):308-9. doi: 10.1042/bst0080308a.
9
[Extraction and purification of the chief glycoprotein of the erythrocyte membrane].
Rev Fr Transfus Immunohematol. 1979 Sep;22(4):329-41. doi: 10.1016/s0338-4535(79)80029-x.
10
Membrane solubilization by detergents, and detergent/protein ratios [proceedings].去污剂对膜的增溶作用以及去污剂与蛋白质的比例[会议论文集]
Biochem Soc Trans. 1979 Feb;7(1):148-50. doi: 10.1042/bst0070148.
引用本文的文献
1
The relationship of detergent-solubilization plasma-membrane components of rabbit alveolar macrophages to an isolated inhibitor of phagocytosis.兔肺泡巨噬细胞的去污剂增溶质膜成分与一种分离出的吞噬作用抑制剂之间的关系。
Biochem J. 1979 May 1;179(2):305-14. doi: 10.1042/bj1790305.
本文引用的文献
1
Microdetermination of plasma phospholipids by trichloroacetic acid precipitation.用三氯乙酸沉淀法微量测定血浆磷脂
J Lab Clin Med. 1950 Jan;35(1):155-60.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.人红细胞无血红蛋白空泡的制备及其化学特性
Arch Biochem Biophys. 1963 Jan;100:119-30. doi: 10.1016/0003-9861(63)90042-0.
4
The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
J Biol Chem. 1959 Aug;234(8):1971-5.
5
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
6
The determination of sugar in blood and spinal fluid with anthrone reagent.用蒽酮试剂测定血液和脑脊液中的糖。
J Biol Chem. 1955 Jan;212(1):335-43.
7
Method for the determination of hexosamines in tissues.组织中己糖胺的测定方法。
J Biol Chem. 1953 Oct;204(2):553-63.
8
Fractionation of the protein components of human erythrocyte membranes.人红细胞膜蛋白成分的分级分离。
J Biol Chem. 1969 Oct 10;244(19):5118-24.
9
Dissolution of erythrocyte membranes in water and comparison of the membrane protein with other structural proteins.红细胞膜在水中的溶解以及膜蛋白与其他结构蛋白的比较。
Proc Natl Acad Sci U S A. 1968 Nov;61(3):1005-12. doi: 10.1073/pnas.61.3.1005.
10
Human erythrocyte membrane sialoglycoproteins: a study of interconversion.人红细胞膜唾液酸糖蛋白：相互转化的研究
Biochem Biophys Res Commun. 1974 Jul 10;59(1):352-60. doi: 10.1016/s0006-291x(74)80214-7.
Zotero 插件