Fibriani Azzania, Naisanu Katerina, Yamahoki Nicholas, Kinanti Denti Rizki
School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia.
School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia.
J Virol Methods. 2025 Jan;331:115062. doi: 10.1016/j.jviromet.2024.115062. Epub 2024 Nov 17.
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is the primary cause of the Coronavirus disease 2019 (COVID-19) pandemic, which affects millions of people worldwide with high levels of infectivity and mortality. However, the antibodies developed for COVID-19 research and diagnostics are still limited. Therefore, in this study, we developed polyclonal immunoglobulin (IgY) antibodies from chicken egg yolk targeting multi-epitope antigen of SARS-CoV-2. After immunizing hens with a SARS-CoV-2 multi-epitope peptide, IgY antibodies were isolated from chicken eggs and further characterized using SDS-PAGE and ELISA. The results showed that the IgY antibodies were successfully isolated from egg yolks. The sandwich ELISA results demonstrated that the isolated IgYs could bind to SARS-CoV-2 antigens, both the multi-epitope peptide and the trimeric Spike. Furthermore, the developed polyclonal antibodies could recognize SARS-CoV-2 in human nasopharyngeal swab samples, even at the lowest concentration (dilution at 1:10000). Thus, it can be concluded that the developed polyclonal IgYs were successfully produced and have the potential to be applied in the development of COVID-19 diagnostics.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是2019冠状病毒病（COVID-19）大流行的主要病因，该疾病以高传染性和高死亡率影响着全球数百万人。然而，用于COVID-19研究和诊断的抗体仍然有限。因此，在本研究中，我们从鸡卵黄中开发了针对SARS-CoV-2多表位抗原的多克隆免疫球蛋白（IgY）抗体。在用SARS-CoV-2多表位肽免疫母鸡后，从鸡蛋中分离出IgY抗体，并使用SDS-PAGE和ELISA进一步表征。结果表明，IgY抗体成功地从蛋黄中分离出来。夹心ELISA结果表明，分离出的IgY能够与SARS-CoV-2抗原结合，包括多表位肽和三聚体刺突蛋白。此外，所开发的多克隆抗体能够识别人类鼻咽拭子样本中的SARS-CoV-2，即使在最低浓度（稀释至1:10000）下也是如此。因此，可以得出结论，所开发的多克隆IgY已成功制备，并具有应用于COVID-19诊断开发的潜力。
