Zhu Qianying, Xia Fei, Chen Zhe, Lin Sen, Zhang Qing, Xue Bingru, Dai Weisi
Department of Clinical Laboratory, The Third Affiliated Hospital of Wenzhou Medical University, Rui'an, 325200, Zhejiang, PR China.
Heliyon. 2024 Oct 30;10(21):e39978. doi: 10.1016/j.heliyon.2024.e39978. eCollection 2024 Nov 15.
To understand the virulence genes and molecular epidemiological characteristics of human-infected strains of in Rui'an, Zhejiang Province, from 2021 to 2022, and to provide a scientific basis for diagnosis, treatment, prevention, and control.
Three blood-borne strains of were analysed by morphological observation, identification, and drug sensitivity tests. We performed polymerase chain reaction (PCR) amplification of their main seven virulence factors and housekeeping genes. This was followed by virulence analysis and multilocus sequence typing. We analysed their relationships with local pathogens from previous years.
Three strains were isolated from the blood samples of three patients. From these, the virulence genotypes demonstrated that the two strains were and +, respectively. The Multilocus sequence typing (MLST) typing results demonstrated that the two strains were and respectively.
The first isolation of in Rui'an was presumed to have a close affinity with the endemic strain in North America. The other strain was an clone, consistent with the endemic strain in Sichuan, and which may have originated from Sichuan. Virulence genotype analysis demonstrated that different virulence genes of the pathogens resulted in different clinical manifestations.
了解2021年至2022年浙江省瑞安市人感染菌株的毒力基因及分子流行病学特征，为诊断、治疗、预防和控制提供科学依据。
对3株血源菌株进行形态学观察、鉴定及药敏试验分析。对其7种主要毒力因子和管家基因进行聚合酶链反应（PCR）扩增。随后进行毒力分析和多位点序列分型。分析它们与前几年当地病原体的关系。
从3例患者的血样中分离出3株菌株。其中，毒力基因型显示这2株菌株分别为 和 +。多位点序列分型（MLST）分型结果显示这2株菌株分别为 和 。
瑞安市首次分离出的 菌株推测与北美地方菌株有密切亲缘关系。另一株为 克隆株，与四川地方菌株一致，可能起源于四川。毒力基因型分析表明，病原体不同的毒力基因导致不同的临床表现。
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