Sapkota Bijaya, Trandem Nina, Fránová Jana, Koloniuk Igor, Blystad Dag-Ragnar, Hamborg Zhibo
Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, Ås, Norway.
Biology Centre CAS, Institute of Plant Molecular Biology, České Budějovice, Czechia.
Front Plant Sci. 2024 Nov 1;15:1441145. doi: 10.3389/fpls.2024.1441145. eCollection 2024.
Raspberry ( L.) is susceptible to aphid-borne viruses. We studied the incidence of four of them - black raspberry necrosis virus (BRNV), raspberry leaf mottle virus (RLMV), raspberry vein chlorosis virus (RVCV), and Rubus yellow net virus (RYNV) - in raspberry plants and aphids in and around Norwegian raspberry crops for three years (2019, 2021, and 2022). Most of the samples were from symptomatic plants. Applying RT-PCR, 274 leaf samples and 107 aphid samples were analyzed. All four viruses were found, but BRNV dominated: it was detected in 93% of the 178 leaf samples with virus and was the only virus that occurred more frequently as a single infection than in co-infections with the other viruses. The old cv. Veten had the highest virus incidence (97%) among the sampled plants, followed by uncultivated raspberry in the boundary vegetation (82%). All aphids identified were and BRNV and/or RLMV was detected in 27% of the aphid samples. Notably, BRNV was detected in 30% of samples, a species not known as a BRNV vector. In subsequent transmission experiments we found that although can acquire BRNV within one hour, it did not transmit the virus to healthy raspberry plants. In contrast, , a known BRNV vector, was able to acquire the virus within one minute and transmit it within one hour of inoculation. Our study will improve the identification and management of BRNV.
树莓（蔷薇科悬钩子属）易感染由蚜虫传播的病毒。我们对挪威树莓种植区及周边的树莓植株和蚜虫中四种此类病毒——黑树莓坏死病毒（BRNV）、树莓叶斑驳病毒（RLMV）、树莓叶脉黄化病毒（RVCV）和悬钩子黄网病毒（RYNV）——的发生率进行了为期三年（2019年、2021年和2022年）的研究。大多数样本来自有症状的植株。应用逆转录聚合酶链反应（RT-PCR）对274份叶片样本和107份蚜虫样本进行了分析。四种病毒均被检测到，但BRNV占主导：在178份带有病毒的叶片样本中有93%检测到了该病毒，并且它是唯一一种单一感染比与其他病毒共同感染更频繁出现的病毒。老品种“Veten”在采样植株中病毒发生率最高（97%），其次是边界植被中的野生树莓（82%）。所有鉴定出的蚜虫均为 ，在27%的蚜虫样本中检测到了BRNV和/或RLMV。值得注意的是，在30%的 样本中检测到了BRNV， 是一种未知的BRNV传播媒介。在随后的传播实验中，我们发现尽管 能够在一小时内获取BRNV，但它并未将病毒传播给健康的树莓植株。相比之下，已知的BRNV传播媒介 能够在一分钟内获取病毒并在接种后一小时内传播病毒。我们的研究将改进BRNV的鉴定和管理。
