Department of Medical Microbiology and Immunology, School of Basic Medicine, Dali University, Dali, Yunnan Province, 671099, PR China - College of Life Sciences, Changchun Sci-Tech University, Shuangyang, Jilin Province, 130600, PR China - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, 225000, PR China.
Guangxi Key Laboratory of Brain and Cognitive Neuroscience, College of Basic Medicine, Guilin Medical University, Guilin, the Guangxi Zhuang Autonomous Region, 541199, PR China.
Parasite. 2024;31:71. doi: 10.1051/parasite/2024071. Epub 2024 Nov 19.
Enterocytozoon bieneusi, a zoonotic pathogen prevalent in both humans and animals, is the most frequently diagnosed microsporidian species in humans and presents significant public health risks. However, data on the prevalence and genotypes of E. bieneusi in farmed minks (Neovison vison) and raccoon dogs (Nyctereutes procyonoides) in China are limited. Therefore, 275 minks (89 from Hebei Province, 57 from Heilongjiang Province, 109 from Liaoning Province, 20 from Shandong Province) and 235 raccoon dogs (114 from Hebei Province, 27 from Heilongjiang Province, 61 from Liaoning Province, 33 from Jilin Province) were examined for the prevalence and genotypes of E. bieneusi through sequence analysis of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi was 18.6% (95/510), with 10.5% (29/275) in farmed minks and 28.1% (66/235) in raccoon dogs. Ten genotypes (CHN-F1, genotype D, Type IV, EbpC, NCF2, NCF5, NCF6, Peru8, Henan V, and MJ5) were identified in minks and raccoon dogs. This study is the first to detect the CHN-F1, NCF2, NCF6, Peru8, and Henan V genotypes in minks and the NCF5, NCF6, and MJ5 genotypes in raccoon dogs. Additionally, the D, Type IV, and Peru8 genotypes, previously identified in humans, were also found in minks and raccoon dogs, suggesting that these animals could be potential sources of human microsporidiosis. These findings expand the understanding of E. bieneusi's host distribution in China and contribute to the prevention of zoonotic E. bieneusi infections among farmed animals.
肠微孢子虫(Enterocytozoon bieneusi)是一种人畜共患病原,在人类和动物中广泛流行,是人类最常诊断出的微孢子虫物种,存在重大公共卫生风险。然而,关于中国养殖水貂(Neovison vison)和貉(Nyctereutes procyonoides)中肠微孢子虫的流行率和基因型的数据有限。因此,通过 rRNA 基因内转录间隔区(ITS)序列分析,检测了 275 只水貂(89 只来自河北省,57 只来自黑龙江省,109 只来自辽宁省,20 只来自山东省)和 235 只貉(114 只来自河北省,27 只来自黑龙江省,61 只来自辽宁省,33 只来自吉林省)中肠微孢子虫的流行率和基因型。肠微孢子虫的总流行率为 18.6%(95/510),养殖水貂为 10.5%(29/275),貉为 28.1%(66/235)。在水貂和貉中鉴定出 10 种基因型(CHN-F1、基因型 D、Type IV、EbpC、NCF2、NCF5、NCF6、秘鲁 8 号、河南 5 号和 MJ5)。本研究首次在水貂中检测到 CHN-F1、NCF2、NCF6、秘鲁 8 号和河南 5 号基因型,在貉中检测到 NCF5、NCF6 和 MJ5 基因型。此外,还在水貂和貉中发现了先前在人类中发现的 D、Type IV 和秘鲁 8 号基因型,表明这些动物可能是人类微孢子虫病的潜在来源。这些发现扩展了对中国肠微孢子虫宿主分布的认识,有助于预防养殖动物中的人畜共患肠微孢子虫感染。