使用51铬释放法来测定葡萄球菌杀白细胞素的细胞毒性作用以及毒素中和对牛白细胞的影响。
Use of 51Cr release to measure the cytotoxic effects of staphylococcal leukocidin and toxin neutralization on bovine leukocytes.
作者信息
Loeffler D A, Schat K A, Norcross N L
出版信息
J Clin Microbiol. 1986 Mar;23(3):416-20. doi: 10.1128/jcm.23.3.416-420.1986.
Leukocidin toxin from Staphylococcus aureus produces specific cytolytic effects on neutrophils and macrophages. The most commonly used method for determination of leukocidin activity is microscopic examination for characteristic morphological changes in toxin-treated cells. The 51Cr release assay was modified to allow quantitation of the cytolytic effects of leukocidin on bovine peripheral blood neutrophils and lymphocytes. Toxin neutralization by serum and milk samples was quantitated by this method. The neutralizing abilities of the various samples were found to correlate with the levels of immunoglobulin G (IgG1) specific for leukocidin. Undiluted normal serum samples, however, were capable of partially preventing the cytotoxic effects of leukocidin. The assay was shown to be an effective means of quantitating the cytotoxic activity of leukocidin on neutrophils as well as demonstrating neutralization of cytotoxicity by milk and serum samples.
金黄色葡萄球菌的杀白细胞素毒素对中性粒细胞和巨噬细胞产生特异性细胞溶解作用。测定杀白细胞素活性最常用的方法是通过显微镜检查毒素处理细胞的特征性形态变化。对51Cr释放试验进行了改进，以定量杀白细胞素对牛外周血中性粒细胞和淋巴细胞的细胞溶解作用。用该方法对血清和乳样中的毒素中和作用进行定量。发现各种样品的中和能力与针对杀白细胞素的免疫球蛋白G（IgG1）水平相关。然而，未稀释的正常血清样品能够部分阻止杀白细胞素的细胞毒性作用。该试验被证明是定量杀白细胞素对中性粒细胞细胞毒性活性以及证明乳样和血清样品对细胞毒性的中和作用的有效手段。
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