Leonardi Giuseppa Rosaria, Aiello Dalia, Di Pietro Chiara, Gugliuzzo Antonio, Tropea Garzia Giovanna, Polizzi Giancarlo, Voglmayr Hermann
Department of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 100, 95123 Catania, Italy University of Catania Catania Italy.
Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030 Vienna, Austria University of Vienna Vienna Austria.
MycoKeys. 2024 Nov 15;110:211-236. doi: 10.3897/mycokeys.110.129228. eCollection 2024.
is an important Mediterranean tree and shrub native to Italy that is also commercially grown as spice and ornamental plant. Field surveys conducted since 2021 in Sicily (Italy) revealed that bay laurel plants in urban and private gardens and nurseries were severely affected by symptoms of stem blight and internal necrosis, which were associated with ambrosia beetle entry holes in the bark and internal wood galleries. The occurring ambrosia beetle was identified as , an invasive wood-boring pest previously reported from Sicily. Investigation of fungi from symptomatic tissues primarily resulted in the isolation of -like colonies. The main symbiont of , , was also isolated from infested plants. Phylogenetic analyses of a combined matrix of ITS, LSU, , , , and gene regions revealed that the isolated -like colonies represent a new fungal species within the genus . Based on both phylogeny and morphology, the new isolated fungus is described as Moreover, two recently described species, and , are transferred to the genus due to the confirmed synonymy of both genera, as supported by molecular phylogenies. Pathogenicity test conducted on potted plants demonstrated that is pathogenic to bay laurel, causing internal necrosis and stem blight. The new species was consistently re-isolated from the symptomatic tissue beyond the inoculation point, thereby fulfilling Koch's postulates. This study represents the first report of a new pathogenic fungus, , causing stem blight and internal necrosis of bay laurel plants and associated with infestation of the invasive ambrosia beetle .
是一种重要的地中海树木和灌木，原产于意大利，也作为香料和观赏植物进行商业种植。自2021年以来在意大利西西里岛进行的实地调查显示，城市和私人花园及苗圃中的月桂树受到茎枯病和内部坏死症状的严重影响，这些症状与树皮上的粉蠹虫蛀孔和内部木质虫道有关。发现的粉蠹虫被鉴定为，这是一种此前在西西里岛有报道的入侵性蛀木害虫。对有症状组织中的真菌进行调查，主要分离出了类似的菌落。的主要共生菌，也从受侵染的植物中分离出来。对ITS、LSU、、、和基因区域的联合矩阵进行系统发育分析表明，分离出的类似菌落代表属内的一个新真菌物种。基于系统发育和形态学，新分离出的真菌被描述为此外，由于两个属的同义性得到分子系统发育的支持，最近描述的两个物种，和，被转移到属。对盆栽植物进行的致病性测试表明，对月桂树具有致病性，会导致内部坏死和茎枯病。新物种始终能从接种点以外的有症状组织中重新分离出来，从而满足了科赫法则。本研究首次报道了一种新的致病真菌，导致月桂树植物茎枯病和内部坏死，并与入侵性粉蠹虫的侵染有关。
