Trauma and Transfusion Medicine Research Center, Department of Surgery, University of Pittsburgh;
Trauma and Transfusion Medicine Research Center, Department of Surgery, University of Pittsburgh.
J Vis Exp. 2024 Nov 8(213). doi: 10.3791/67214.
Microfluidics incorporate physiologically relevant substrates and flows that mimic the vasculature and are, therefore, a valuable tool for studying aspects of thrombosis and hemostasis. At high-shear environments simulating arterial flow, a microfluidic assay facilitates the study of platelet function, as platelet-rich thrombi form in a localized stenotic region of a flow channel. Utilizing devices that allow for small sample volume can additionally aid in evaluating platelet function under flow from volume-limited patient samples or animal models. Studying trauma patient samples or samples following platelet product transfusion may aid in directing therapeutic strategies for patient populations in which platelet function is critical. Effects of platelet inhibition via pharmacological agents can also be studied in this model. The objective of this protocol is to establish a microfluidic platform that incorporates physiologic flow, biological surfaces, and relevant hemostatic mechanisms to assess platelet function with implications for the study of trauma induced coagulopathy and transfusion medicine.
微流控技术整合了生理相关的基质和流场，模拟了脉管系统，因此是研究血栓形成和止血的一个有价值的工具。在模拟动脉血流的高剪切环境中，微流控分析可以促进血小板功能的研究，因为富含血小板的血栓会在流道的局部狭窄区域形成。利用允许小样本量的设备，还可以帮助评估来自体积有限的患者样本或动物模型的流动条件下的血小板功能。研究创伤患者样本或血小板制品输注后的样本可能有助于为血小板功能至关重要的患者群体指导治疗策略。通过药理学药物抑制血小板的效果也可以在这种模型中进行研究。本方案的目的是建立一个微流控平台，整合生理流场、生物表面和相关止血机制，以评估血小板功能，这对创伤性凝血病和输血医学的研究具有重要意义。
