小鼠合子期雌原核/细胞质比率与染色体数目之间的相关性：对辅助生殖技术中染色体非整倍体评估的意义
Correlation between female pronuclear/cytoplasmic ratio and number of chromosomes in mouse zygotic stage: implications for aneuploidy assessment in ART.
作者信息
Xiao Wei, Akao Sakura, Otsuki Junko
机构信息
Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 1-1-1 Tsushimanaka, Kita, Okayama, 700-8530, Japan.
Faculty of Agriculture, Okayama University, 1-1-1 Tsushimanaka, Kita, Okayama, 700-8530, Japan.
出版信息
J Assist Reprod Genet. 2025 Jan;42(1):85-95. doi: 10.1007/s10815-024-03312-5. Epub 2024 Nov 25.
PURPOSE
The objective of this study was to investigate the correlation between the pronuclear/cytoplasmic (PN/C) ratio and the number of chromosomes in mouse zygotes to understand the implications of pronuclear size regulation in early embryonic development.
METHODS
A combination of enucleation and aggregated chromosomes/chromatin (AC) transfer was utilized to create oocytes with varying numbers of chromosomes. Time-lapse imaging and immunofluorescence staining were employed to analyze pronuclear dynamics and chromosomal configurations.
RESULTS
Higher chromosome numbers correspond to a larger PN/C ratio. Oocytes with a higher number of chromosomes exhibited larger pronuclei.
CONCLUSION
The study underscores the complexity of pronuclear size regulation and its correlation with the number of chromosomes. The findings suggest potential applications in ART, where assessing the PN/C ratio could serve as a biomarker for zygote quality and aneuploidy.
目的
本研究的目的是调查小鼠受精卵中原核/细胞质（PN/C）比率与染色体数量之间的相关性，以了解原核大小调控在早期胚胎发育中的意义。
方法
采用去核与聚集染色体/染色质（AC）转移相结合的方法，创建具有不同染色体数量的卵母细胞。利用延时成像和免疫荧光染色分析原核动态和染色体构型。
结果
染色体数量越多，PN/C比率越大。染色体数量较多的卵母细胞表现出更大的原核。
结论
该研究强调了原核大小调控的复杂性及其与染色体数量的相关性。研究结果表明，在辅助生殖技术（ART）中可能具有潜在应用，评估PN/C比率可作为受精卵质量和非整倍体的生物标志物。
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