健康个体、阻塞性睡眠呼吸暂停患者和使用持续气道正压通气治疗患者的肠道微生物组变化：全基因组宏基因组分析。

Gut microbiota changes in healthy individuals, obstructive sleep apnea patients, and patients treated using continuous positive airway pressure: a whole-genome metagenomic analysis.

机构信息

Department of Pulmonary and Critical Care Medicine of the Second Medical Center and National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.

Department of Vasculocardiology, Second Medical Center of Chinese, PLA General Hospital, Beijing, 100853, China.

出版信息

Sleep Breath. 2024 Nov 26;29(1):11. doi: 10.1007/s11325-024-03185-z.

DOI:10.1007/s11325-024-03185-z

PMID:39589660

Abstract

PURPOSE

This study investigated variations in gut microbiota among severe obstructive sleep apnea (OSA) patients and changes in gut microbiota after continuous positive airway pressure (CPAP) treatment.

METHOD

From November 2020 to August 2021, laboratory-based polysomnography (PSG) was used to measure sleep parameters in healthy controls, severe OSA patients, and severe OSA patients treated with CPAP for three months. A fully automated biochemical analyzer was used to evaluate routine blood tests and biochemical indicators. Whole-genome metagenomic analysis was used to determine the microbial composition of gut samples from all participants. The relationships between gut microbiota and hypertension were examined using correlation analysis.

RESULT

The relative abundances of Bacteroides, Firmicutes, and Parabacteroides were significantly lower at the species level. Enterobacterales and Turicibacter were significantly higher in participants with severe OSA than healthy controls. Negative correlations were identified between Bacteroides coprocola and systolic blood pressure (SBP) (r = - 0.710, P = 0.003) and diastolic blood pressure (DBP) (r = - 0.615, P = 0.015). Conversely, a positive correlation was found between Escherichia coli and SBP (r = 0.568, P = 0.027).

CONCLUSION

The metabolic pathways and gut microbiota differed significantly between the control group and individuals with severe OSA. Additionally, CPAP therapy substantially changed the metabolic pathways and gut microbial composition among patients diagnosed with severe OSA. Correlation analysis further revealed a strong association between Escherichia coli, Bacteroides coprocola, and blood pressure levels.

摘要

目的

本研究旨在探讨严重阻塞性睡眠呼吸暂停（OSA）患者肠道微生物群的变化，以及持续气道正压通气（CPAP）治疗后肠道微生物群的变化。

方法

2020 年 11 月至 2021 年 8 月，采用实验室基础多导睡眠图（PSG）测量健康对照组、严重 OSA 患者和严重 OSA 患者接受 CPAP 治疗 3 个月后的睡眠参数。使用全自动生化分析仪评估常规血液检查和生化指标。采用全基因组宏基因组分析确定所有参与者肠道样本的微生物组成。采用相关分析研究肠道微生物群与高血压之间的关系。

结果

在物种水平上，拟杆菌、厚壁菌门和拟杆菌的相对丰度显著降低。与健康对照组相比，严重 OSA 患者的肠杆菌科和 Turicibacter 明显升高。Bacteroides coprocola 与收缩压（SBP）（r = -0.710，P = 0.003）和舒张压（DBP）（r = -0.615，P = 0.015）呈负相关。相反，大肠杆菌与 SBP 呈正相关（r = 0.568，P = 0.027）。