College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2024 Nov 20;25(22):12450. doi: 10.3390/ijms252212450.
Leizhou goats can be classified into tall and short types based on their size and habits. The tall Leizhou goats are well-suited for grazing management due to their robust physique, while the dwarf types are smaller, grow rapidly, and are more appropriate for feeding management systems. In this study, whole-genome resequencing was conducted to identify genomic variants in 15 Tall-legged (TL) and 15 Short-legged (SL) Leizhou goats, yielding 8,641,229 high-quality SNPs in the Leizhou goat genome. Phylogenetic tree and principal component analyses revealed obvious genetic differentiation between the two groups. Fst and θπ analyses identified 420 genes in the TL group and 804 genes in the SL group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that the phosphatidylinositol signaling system is associated with growth and development. Additionally, Genome-Wide Association Study (GWAS) analysis identified eight genes linked to leg length, including and . Notably, the NC_030818.1 (g.53666634T > C) variant was significantly associated with leg length traits, where the CC genotype was linked to shorter legs and the TT genotype to longer legs. This study identifies candidate genes and molecular markers, serving as a reference point for breeding and genetic improvement efforts in Leizhou goats and other goat breeds.
雷州羊可以根据体型和习性分为高腿型和短腿型。高腿型雷州羊体质健壮，适合放牧管理，而矮腿型雷州羊体型较小，生长迅速，更适合饲养管理系统。本研究通过全基因组重测序，在 15 只长腿（TL）和 15 只短腿（SL）雷州羊中鉴定了基因组变异，在雷州羊基因组中获得了 8641229 个高质量 SNP。系统进化树和主成分分析显示两组间存在明显的遗传分化。Fst 和θπ分析在 TL 组中鉴定了 420 个基因，在 SL 组中鉴定了 804 个基因。基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析表明，磷酸肌醇信号系统与生长和发育有关。此外，全基因组关联研究（GWAS）分析鉴定了与腿长相关的 8 个基因，包括和。值得注意的是，NC_030818.1（g.53666634T > C）变异与腿长性状显著相关，CC 基因型与腿短相关，TT 基因型与腿长相关。本研究鉴定了候选基因和分子标记，为雷州羊和其他山羊品种的选育和遗传改良提供了参考。
