具有产蛋下降的加拿大禽群中独特型传染性支气管炎病毒（IBV）变异株的核苷酸和氨基酸分析。

Nucleotide and Amino Acid Analyses of Unique Infectious Bronchitis Virus (IBV) Variants from Canadian Poultry Flocks with Drop in Egg Production.

机构信息

Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, 3330 Hospital Drive, NW, Calgary, AB T2N 4N1, Canada.

Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt.

出版信息

Genes (Basel). 2024 Nov 17;15(11):1480. doi: 10.3390/genes15111480.

DOI:10.3390/genes15111480

PMID:39596680

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11593648/

Abstract

BACKGROUND/OBJECTIVES: Infectious bronchitis (IB) is a highly infectious avian disease caused by the infectious bronchitis virus (IBV). The disease causes lesions mainly in the respiratory, reproductive, and renal systems and has a significant economic impact on the poultry industry worldwide.

METHODS

We discovered two unique IBV isolates (T-62: PP737794.1 and CL-61: PP783617.1) circulating in Canada and molecularly characterized them.

RESULTS

The phylogenetic analysis revealed that the IBV isolates belong to genotype I and fall between lineages 25 and 7. Further analysis of the T-62 IBV isolate indicated that it is a potential recombinant of the Iowa state isolate (IA1162/2020-MW) and that the CL-61 strain of the IBV is also a recombinant IBV with the Connecticut (Conn) vaccine strain as its major parent. The S1 glycoprotein of the CL-61 and T-62 strains of the IBV had 85.7% and 73.2% amino acid (aa) identities respectively compared to the Conn vaccine strain. There were 67 and 129 aa substitutions among the S1 glycoprotein of the CL-61 and T-62 strains of the IBV compared to the Conn vaccine, respectively. Importantly, two and nineteen of these aa variations were in hypervariable regions 1 (HVR1) and HVR3. Finally, the two IBV isolates possessed a higher affinity for the sialic acid ligand compared to the DMV/1639 and Mass/SES IBV strains.

CONCLUSIONS

Genetic recombination in the IBV results in the continual emergence of new variants, posing challenges for the poultry industry. As indicated by our analyses, live attenuated vaccine strains play a role in the genetic recombination of the IBV, resulting in the emergence of variants.

摘要

背景/目的：传染性支气管炎（IB）是一种由传染性支气管炎病毒（IBV）引起的高度传染性禽类疾病。该疾病主要引起呼吸道、生殖和肾脏系统的病变，对全球家禽业造成重大经济影响。

方法

我们发现了两种在加拿大流行的独特 IBV 分离株（T-62：PP737794.1 和 CL-61：PP783617.1），并对其进行了分子特征分析。

结果

系统进化分析表明，IBV 分离株属于基因型 I，位于 25 与 7 两个谱系之间。对 T-62 IBV 分离株的进一步分析表明，它是爱荷华州分离株（IA1162/2020-MW）的潜在重组株，而 CL-61 株的 IBV 也是以康州（Conn）疫苗株为主要亲本的重组 IBV。CL-61 和 T-62 株的 IBV S1 糖蛋白与 Conn 疫苗株相比，分别有 85.7%和 73.2%的氨基酸（aa）同一性。CL-61 和 T-62 株的 IBV S1 糖蛋白与 Conn 疫苗株相比，分别有 67 和 129 个 aa 取代。重要的是，这两种 aa 变异中有两个和十九个位于高度可变区 1（HVR1）和 HVR3。最后，与 DMV/1639 和 Mass/SES IBV 株相比，这两种 IBV 分离株对唾液酸配体具有更高的亲和力。