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中国南方的日本真牡蛎基因组染色体水平组装。

Chromosome-level genome assembly of the Suminoe oyster Crassostrea ariakensis in south China.

机构信息

Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.

Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266100, China.

出版信息

Sci Data. 2024 Nov 27;11(1):1296. doi: 10.1038/s41597-024-04145-8.

DOI:10.1038/s41597-024-04145-8
PMID:39604404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11603178/
Abstract

The Suminoe oyster Crassostrea ariakensis (Fujita, 1913) is one of the most important ecological and fishery bivalve mollusks with a worldwide distribution. Here, we reported an improved high-quality chromosomal-level genome assembly of C. ariakensis inhabiting the South China Sea, using Nanopore technology, Illumina sequencing, and high-throughput chromosomal conformation capture analysis. The assembled genome size is 631.73 Mb, with contig N50 length of 5.36 Mb and scaffold N50 length of 61.15 Mb, and is assigned to 10 chromosomes. A total of 29,357 protein-coding genes are predicted, 96.68% of which are functionally annotated. The genome contains 347.11 Mb (54.94%) of repetitive elements and 1130 noncoding RNAs. This improved genome assembly of south C. ariakensis is an important resource for understanding oyster diversity and evolution, and provides insights into genetic improvement, protection and management of oyster resource.

摘要

日本囊对虾(Crassostrea ariakensis)是分布广泛的具有重要生态和渔业价值的双壳贝类之一。本研究采用纳米孔测序、Illumina 测序和高通量染色体构象捕获分析技术,对栖息在中国南海的日本囊对虾进行了高质量的染色体水平基因组组装。组装得到的基因组大小为 631.73Mb,contig N50 长度为 5.36Mb,scaffold N50 长度为 61.15Mb,基因组被分配到 10 条染色体上。共预测到 29357 个蛋白质编码基因,其中 96.68%具有功能注释。基因组包含 347.11Mb(54.94%)的重复序列和 1130 个非编码 RNA。该研究为深入研究牡蛎多样性和进化提供了重要资源,同时也为牡蛎资源的遗传改良、保护和管理提供了新的思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/082e/11603178/7b59e99a74ad/41597_2024_4145_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/082e/11603178/f8a30f2b5d89/41597_2024_4145_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/082e/11603178/7b59e99a74ad/41597_2024_4145_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/082e/11603178/f8a30f2b5d89/41597_2024_4145_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/082e/11603178/7b59e99a74ad/41597_2024_4145_Fig2_HTML.jpg

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