Wang Baolong, Ma Pengfei, Liu Mingyuan, Huang Ruiping, Qiu Zhujun, Pan Lanlan, Wang Jinghan, Liu Ying, Zhang Qian
Dalian Ocean University, Dalian, 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, 116023, China.
Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, 116023, China.
Environ Res. 2025 Jan 15;265:120342. doi: 10.1016/j.envres.2024.120342. Epub 2024 Nov 26.
Cultivating microalgae for the remediation of aquaculture wastewater provides a promising solution for pollution control. However, the economic viability of this approach faces challenges due to the high costs associated with microalgal biomass harvesting. This study aims to address this issue by immobilizing microalgae onto coral velvet carriers, enhancing the efficiency of biomass recovery. Four types of microalgae were screened: Chlorella sp., Isochrysis galbana, Chaetoceros sp., and Nannochloropsis sp. Among them, Isochrysis galbana exhibited the best self sedimentation rate, achieving a self sedimentation rate of 94.36%. Chlorella sp. demonstrated the best denitrification rate, with a nitrate removal rate of 100% and an inorganic nitrogen removal rate of 79.13%. In addition, this study found that extracellular polymeric substances(EPS) affects the self-settling performance of microalgae, and the results emphasize the key role of tightly-bound EPS(TB-EPS) content in determining self settling efficiency. Furthermore,the assessments of the purification of simulated aquaculture wastewater were conducted, comparing the outcomes of co-cultivation with mono-culture. The co-cultivation strategy showed exceptional efficacy, achieving a 100% removal rate for NO-N by the 5th day. In contrast, mono-cultures of Chlorella sp. and Isochrysis galbana showed removal rates of 77.76% and 45.72%, respectively, at the same interval. Applying of the co-cultivation microalgal biofilm to treat the actual aquaculture wastewater showed remarkable denitrification performance, attaining a 100% removal rate for NO-N by the 7th day. The study proposes the co-cultivation of Chlorella sp. and Isochrysis galbana for treating aquaculture wastewater and explores the potential application of immobilization technology to remove nitrogen-containing pollutants.
培养微藻用于修复水产养殖废水为污染控制提供了一个有前景的解决方案。然而，由于微藻生物质收获相关的高成本，这种方法的经济可行性面临挑战。本研究旨在通过将微藻固定在珊瑚绒载体上来解决这个问题，提高生物质回收效率。筛选了四种微藻：小球藻属、等鞭金藻、角毛藻属和微拟球藻属。其中，等鞭金藻表现出最佳的自沉降率，自沉降率达到94.36%。小球藻属表现出最佳的反硝化率，硝酸盐去除率为100%，无机氮去除率为79.13%。此外，本研究发现胞外聚合物（EPS）影响微藻的自沉降性能，结果强调了紧密结合的EPS（TB-EPS）含量在决定自沉降效率中的关键作用。此外，进行了模拟水产养殖废水净化的评估，比较了共培养与单培养的结果。共培养策略显示出卓越的效果，到第5天NO-N去除率达到100%。相比之下，小球藻属和等鞭金藻的单培养在相同时间段的去除率分别为77.76%和45.72%。应用共培养微藻生物膜处理实际水产养殖废水显示出显著的反硝化性能，到第7天NO-N去除率达到100%。该研究提出将小球藻属和等鞭金藻共培养用于处理水产养殖废水，并探索固定化技术去除含氮污染物的潜在应用。
