从丹磺酰基修饰的生物膜破坏剂到β-环糊精优化的多功能超分子纳米囊泡：它们在植物细菌性疾病治疗方面的改进。

From dansyl-modified biofilm disruptors to β-cyclodextrin-optimized multifunctional supramolecular nanovesicles: their improved treatment for plant bacterial diseases.

机构信息

State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guiyang, 550025, China.

出版信息

J Nanobiotechnology. 2024 Nov 28;22(1):739. doi: 10.1186/s12951-024-03028-9.

DOI:10.1186/s12951-024-03028-9

PMID:39609837

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11603638/

Abstract

BACKGROUND

Bacterial diseases caused by phytopathogenic Xanthomonas pose a significant threat to global agricultural production, causing substantial economic losses. Biofilm formation by these bacteria enhances their resistance to environmental stressors and chemical treatments, complicating disease control. The key to overcoming this challenge lies in the development of multifunctional green bactericides capable of effectively breaking down biofilm barriers, improving foliar deposition properties, and achieving the control of bacterial diseases.

RESULTS

We have developed a kind of innovative green bactericide from small-molecule conception to eco-friendly supramolecular nanovesicles (DaPA8@β -CD) by host-guest supramolecular technology. These nanoscale assemblies demonstrated the ability to inhibit and eradicate biofilm formation, while also promoted foliar wetting and effective deposition properties, laying the foundation for improving agrochemical utilization. Studies revealed that DaPA8@β -CD exhibited significant biofilm inhibition (78.66% at 7.0 µ g mL) and eradication (83.50% at 25.0 µ g mL), outperforming DaPA8 alone (inhibition: 59.71%, eradication: 66.79%). These nanovesicles also reduced exopolysaccharide formation and bacterial virulence. In vivo experiments showed enhanced control efficiency against citrus bacterial canker (protective: 78.04%, curative: 50.80%) at a low dose of 200 µ g mL, superior to thiodiazole-copper-20%SC and DaPA8 itself.

CONCLUSION

This study demonstrates the potential of DaPA8@β -CD nanovesicles as multifunctional bactericides for managing Xanthomonas -induced plant diseases, highlighting the advantages of using host-guest supramolecular technology to enhance agrochemical bioavailability.

摘要

背景

植物病原黄单胞菌引起的细菌性疾病对全球农业生产构成重大威胁，造成巨大的经济损失。这些细菌形成生物膜会增强其对环境胁迫和化学处理的抵抗力，从而使疾病控制变得更加复杂。克服这一挑战的关键在于开发多功能绿色杀菌剂，这些杀菌剂能够有效地破坏生物膜屏障，改善叶面沉积特性，并实现对细菌病害的控制。

结果

我们通过主客体超分子技术从小分子概念到环保超分子纳米囊（DaPA8@β-CD）开发了一种创新的绿色杀菌剂。这些纳米级组装体表现出抑制和根除生物膜形成的能力，同时还促进了叶面润湿和有效沉积特性，为提高农用化学品的利用奠定了基础。研究表明，DaPA8@β-CD 对生物膜的抑制率为 78.66%（在 7.0 µg mL 时），根除率为 83.50%（在 25.0 µg mL 时），优于单独的 DaPA8（抑制率为 59.71%，根除率为 66.79%）。这些纳米囊还减少了胞外多糖的形成和细菌的毒力。体内实验表明，在低剂量 200 µg mL 时，对柑橘溃疡病的防治效果增强（保护：78.04%，治疗：50.80%），优于噻二唑铜 20%SC 和 DaPA8 本身。