Augustine J L, Renshaw H W
Am J Vet Res. 1986 Apr;47(4):713-5.
Several inanimate surfaces (eg, plastic, wood, and steel) and particulate fomites (eg, wood shavings, hay, straw, and feces), common to the environment of confined small ruminants, were inoculated with Corynebacterium pseudotuberculosis in axenic purulent exudate that had been surgically removed from a naturally occurring case of caprine caseous lymphadenitis. Each inoculated fomite was incubated at 37, 22, and 4 C, and the length of time that C pseudotuberculosis survived was determined by isolation of bacteria from the fomite. The organism remained viable longer when caseous lymphadenitis abscess contents were mixed with particulate fomites than when spread on surfaces. Incubation at lower temperatures generally extended the survival potential of C pseudotuberculosis. Depending on the particulate fomite and the incubation temperature, viable C pseudotuberculosis organisms were isolated for mean periods ranging from 7 to 55 days, whereas recovery of bacteria from surfaces varied from 1 to 8 days.
从自然发生的山羊干酪性淋巴结炎病例手术切除的无菌脓性渗出物中获取伪结核棒状杆菌，接种于圈养小反刍动物环境中常见的几种无生命表面（如塑料、木材和钢铁）以及颗粒污染物（如木屑、干草、稻草和粪便）上。将每个接种的污染物分别在37℃、22℃和4℃下孵育，并通过从污染物中分离细菌来确定伪结核棒状杆菌存活的时间。当干酪性淋巴结炎脓肿内容物与颗粒污染物混合时，该微生物存活的时间比涂抹在表面时更长。在较低温度下孵育通常会延长伪结核棒状杆菌的存活期。根据颗粒污染物和孵育温度的不同，从颗粒污染物中分离出存活的伪结核棒状杆菌的平均时间为7至55天，而从表面分离细菌的时间则为1至8天。
