Thanh Dinh Duy, Bich-Ngoc Nguyen, Paques Cécile, Christian Aurélie, Herkenne Stéphanie, Struman Ingrid, Muller Marc
Lab. for Organogenesis and Regeneration, GIGA-Institute, Université de Liège, Liège, 4000, Belgium.
Department of Cell Biology, Faculty of Biology, VNU University of Science, Hanoi, 100000, Vietnam.
Sci Rep. 2024 Dec 5;14(1):30367. doi: 10.1038/s41598-024-82076-5.
Tartrazine (E102) is a controversial coloring agent whose potential impacts on human health are not fully understood. Our study reveals the vascular disrupting effects of tartrazine (TTZ) on developing zebrafish embryos in vivo and on human umbilical vein endothelial cells in vitro. The dye was shown to cause dose-dependent hemorrhages in zebrafish embryos. Analyzing transgenic zebrafish harboring fluorescent endothelial cells revealed that TTZ treatment disrupted cell organization into vessels in both the sub-intestinal vein and the brain area. Assays on human umbilical vein endothelial cells demonstrated that TTZ inhibited endothelial proliferation, tube formation, and migration in a dose-dependent manner. Taken together, our results indicate for the first time that TTZ can affect endothelial cell properties, possibly by disrupting Rho family GTPase pathways which control the cytoskeleton. Our finding provides a credible explanation for many reported human health impacts and offers prospective applications for biomedicine.
柠檬黄（E102）是一种存在争议的着色剂，其对人类健康的潜在影响尚未完全明确。我们的研究揭示了柠檬黄（TTZ）在体内对发育中的斑马鱼胚胎以及在体外对人脐静脉内皮细胞的血管破坏作用。该染料在斑马鱼胚胎中会导致剂量依赖性出血。对带有荧光内皮细胞的转基因斑马鱼进行分析发现，TTZ处理会破坏肠下静脉和脑区血管中的细胞组织。对人脐静脉内皮细胞的检测表明，TTZ以剂量依赖性方式抑制内皮细胞增殖、管腔形成和迁移。综上所述，我们的结果首次表明，TTZ可能通过破坏控制细胞骨架的Rho家族GTP酶途径来影响内皮细胞特性。我们的发现为许多已报道的对人类健康的影响提供了可信的解释，并为生物医学提供了潜在应用。
