植物线粒体中杜罗氢醌氧化酶活性与抗氰电子传递途径末端氧化步骤之间的区分。

Discrimination between duroquinol oxidase activity and the terminal oxidation step of the cyanide-resistant electron transport pathway of plant mitochondria.

作者信息

Rustin P, Alin M F, Lance C

出版信息

Biochem Biophys Res Commun. 1986 Mar 13;135(2):677-82. doi: 10.1016/0006-291x(86)90046-x.

Abstract

Comparison of the cyanide-resistant duroquinol oxidase activity of sub-mitochondrial particles from Arum maculatum L. with their ability to carry out a cyanide-resistant oxidation of NADH and succinate shows that heat-inactivation of the duroquinol oxidase activity does not proportionally affect NADH and succinate oxidation. Moreover, 1 microM antimycin inhibits duroquinol oxidase activity by 50% while not decreasing the rates of NADH and succinate oxidation. Therefore, the cyanide-resistant electron transport does not appear to be mediated by a "duroquinol oxidase", and a convincing proof of the existence of a specific protein acting as a cyanide-resistant oxidase in plant mitochondria is still lacking.

摘要

对海芋（Arum maculatum L.）亚线粒体颗粒的抗氰化物杜罗醌醇氧化酶活性与其对NADH和琥珀酸进行抗氰化物氧化能力的比较表明，杜罗醌醇氧化酶活性的热失活对NADH和琥珀酸氧化的影响并不成比例。此外，1微摩尔抗霉素可抑制杜罗醌醇氧化酶活性50%，但不会降低NADH和琥珀酸的氧化速率。因此，抗氰化物电子传递似乎不是由“杜罗醌醇氧化酶”介导的，并且仍然缺乏关于植物线粒体中存在一种作为抗氰化物氧化酶的特定蛋白质的令人信服的证据。