Effect of quinine on mitochondrial K+ and Mg++ flux.

Quinine decreases rates of unidirectional K+ flux into and out of respiring rat liver mitochondria. K+ efflux is more sensitive to quinine than K+ influx. The data are consistent with the proposal that two separate mechanisms may mediate K+ influx, only one of which is sensitive to quinine. Effects on K+ flux of the stereoisomer quinidine are similar to effects of quinine. The smaller quinuclidine causes at most a slight inhibition of K+ efflux under the same conditions. Mg++ flux exhibits a pattern of inhibition by quinine similar to that of K+ flux. Mg++ efflux is more sensitive to quinine than is Mg++ influx. These and earlier findings indicate marked similarities between liver mitochondrial transport mechanisms for K+ and Mg++.