Leder O
Microsc Acta Suppl. 1979(3):129-38.
Adequate methods of sampling are by far the most neglected procedures in stereological work. This is true of both theory and practice. Indeed, it is often forgotten that Delesse not only introduced the stereological method, but also specified the necessary conditions for its use. To help make good this deficiency methods of stereological sampling are reviewed. In particular, the ratio estimate is discussed and examples are given. Estimation of the percentages of each cell class in populations of blood cells and cells from vaginal smears have problems in common. In most cases the bias of the ratio estimate is trivial. The formula for approximate calculation of the standard error of the ratio estimate requires more sampling units than most people realize. In addition, in artifical smears, a more even distribution of the cells is recommended.
到目前为止,适当的抽样方法是体视学工作中最被忽视的步骤。理论和实践都是如此。事实上,人们常常忘记,德莱塞不仅引入了体视学方法,还规定了其使用的必要条件。为了弥补这一不足,本文对体视学抽样方法进行了综述。特别讨论了比率估计,并给出了示例。血细胞群体和阴道涂片细胞中各细胞类别的百分比估计存在共同问题。在大多数情况下,比率估计的偏差很小。比率估计标准误差的近似计算公式所需的抽样单位比大多数人意识到的要多。此外,在人工涂片中,建议细胞分布更均匀。