谷氨酰胺转运至大鼠肠系膜淋巴细胞的过程。

The transport of glutamine into rat mesenteric lymphocytes.

作者信息

Ardawi M S, Newsholme E A

出版信息

Biochim Biophys Acta. 1986 Apr 25;856(3):413-20. doi: 10.1016/0005-2736(86)90131-8.

Abstract

The transport of glutamine into isolated rat mesenteric lymphocytes was studied. This transport appears to be dependent upon the Na+ gradient. The Km for glutamine transport was about 1.0 mM. A large number of amino acids were shown to inhibit the rate of transport of both serine and glutamine into lymphocytes. The transport of glutamine was competitively inhibited by serine and that for serine was similarly inhibited by glutamine. In contrast, histidine and 2-(methylamino)isobutyrate inhibited the transport of both serine and glutamine noncompetitively. It is concluded that glutamine is transported into rat mesenteric lymphocytes by a process similar to System ASC described for other cells.

摘要

对谷氨酰胺转运至分离的大鼠肠系膜淋巴细胞的过程进行了研究。这种转运似乎依赖于钠离子梯度。谷氨酰胺转运的米氏常数约为1.0毫摩尔。大量氨基酸被证明可抑制丝氨酸和谷氨酰胺转运至淋巴细胞的速率。谷氨酰胺的转运受到丝氨酸的竞争性抑制，而丝氨酸的转运也受到谷氨酰胺的类似抑制。相比之下，组氨酸和2-（甲氨基）异丁酸对丝氨酸和谷氨酰胺的转运均有非竞争性抑制作用。得出的结论是，谷氨酰胺通过一种类似于其他细胞中所描述的系统ASC的过程转运至大鼠肠系膜淋巴细胞。