Atashi Amir, Jafaripour Leila, Froughi Kobra, Behzadifard Mahin
Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Department of Anatomy, School of Medical Sciences, Dezful University of Medical Sciences, Dezful, Iran.
Ann Med Surg (Lond). 2024 Oct 16;86(12):7120-7124. doi: 10.1097/MS9.0000000000002244. eCollection 2024 Dec.
BACKGROUND/AIM: B19 virus (B19V) is a single-strand DNA virus that has specific tropism to erythroid progenitor cells (EPCs). The virus enters the cells via P antigen and coreceptors and induces infection and cell apoptosis. GATA1 has a high expression in EPC and is a critical transcription factor for the cells development and differentiation. As human EPCs are the main target of the virus infection that have high expression of GATA-1 as the critical transcription factor, the aim of this study was to investigate the effect of GATA1 cotransfection with B19V genome on the expression of the viral mRNAs in HEK293 as nonpermissive cell line to the virus that had no mRNA expression of GATA-1.
HEK293 cells were transfected with pHI0 plasmid containing the B19V genome and the plasmid of the GATA1 genome. The quantity of B19V mRNAs (NS1, 7.5 kDa, and 11 kDa) expression was evaluated after 24 h of transfection.
The results showed a statistically significant increase in fold change expression of (NS1 ∽12.3, VP1 ∽27.6, 11kb protein ∽38) in cotransfected cells with GATA1 and B19 plasmids compare to control group (<0.05).
This research showed transfected cells with GATA1 had elevation in the expression of the B19V genes mRNAs in a nonpermissive cell. This result may show the role of GATA1 as a critical transcription factor in support of the virus infection in EPCs. This suggests that GATA1 may potentially sport B19V replication or gene expression.
背景/目的：B19病毒（B19V）是一种单链DNA病毒，对红系祖细胞（EPCs）具有特异性嗜性。该病毒通过P抗原和共受体进入细胞，诱导感染和细胞凋亡。GATA1在EPC中高表达，是细胞发育和分化的关键转录因子。由于人类EPC是病毒感染的主要靶标，且高表达GATA-1作为关键转录因子，本研究旨在探讨将GATA1与B19V基因组共转染对HEK293细胞（该病毒的非允许细胞系，且无GATA-1 mRNA表达）中病毒mRNA表达的影响。
用含有B19V基因组的pHI0质粒和GATA1基因组质粒转染HEK293细胞。转染24小时后评估B19V mRNA（NS1、7.5 kDa和11 kDa）的表达量。
结果显示，与对照组相比，共转染GATA1和B19质粒的细胞中（NS1∽12.3、VP1∽27.6、11kb蛋白∽38）的倍数变化表达有统计学意义的增加（<0.05）。
本研究表明，在非允许细胞中，转染GATA1的细胞中B19V基因mRNA的表达升高。这一结果可能表明GATA1作为关键转录因子在支持EPCs中的病毒感染方面的作用。这表明GATA1可能潜在地促进B19V的复制或基因表达。
