Kelner M J, Alexander N M
Clin Chem. 1985 Jan;31(1):112-4.
Urinary myoglobin (Mr = 16 500) is readily separated from hemoglobin (Mr = 64 000) by centrifugation through a microconcentrator membrane with a 30 000-Da cutoff. Myoglobin, but not hemoglobin, will pass through the membrane, after which each fraction may be separately analyzed by spectrophotometry. This technique is advantageous over direct spectrophotometry of urine because it is not restricted to analyzing the oxy forms of these hemoproteins and obviates the difficulty of discriminating small (3 nm) differences in their spectra. In addition, this method of separation is more complete than that attained by differential "salting out" with ammonium sulfate, and it is simpler than gel filtration or ultrafiltration under reduced pressure.
通过使用截留分子量为30000道尔顿的微浓缩器膜进行离心，可轻松将尿肌红蛋白（分子量 = 16500）与血红蛋白（分子量 = 64000）分离。肌红蛋白会透过该膜，而血红蛋白则不会，之后每个组分都可通过分光光度法单独进行分析。与直接对尿液进行分光光度法相比，该技术具有优势，因为它不限于分析这些血红素蛋白的氧合形式，并且避免了区分它们光谱中微小（3纳米）差异的困难。此外，这种分离方法比用硫酸铵进行分级“盐析”更彻底，并且比减压凝胶过滤或超滤更简单。
