Santini G F, Crovatto M, Chiaradia V, Marchi M P
Quad Sclavo Diagn. 1979 Mar;15(1):78-102.
We have attended to a comparative research between two commercial microsystems: Enterotube and Minitek in order identify the Enterobacteriaceae and a reference system given by the combination of the usual macromethods already used in our laboratory. We have examined 401 bacterial cultures of Gram bacillus which we trought to belong to Enterobacteriums, coming from clinical material (excrements, urine, pharyngeal swabs, vaginal swabs, urethral swabs and espectoration) we have received for the bacteriological diagnosis. 390 of 401 cultures have shown to be Enterobacteriums. The biochemical reactions they have given show that the Enterotube and the Minitek have, with the usual system a good accordance for the following tests: dextrose (acid and gaz) lysin and ornithine decarboxylase, production of H2S and indole, phenylalanine deaminase and urease; while we have some statistically significant discordances for the fermenting of lactose and the use of citrate. We have also significant discordances E/C for the fermenting of dulcitole while the ones of Minitek are acceptables. The notes recommend the use, in the specialized bacteriological laboratories, of the conventional tests.
我们对两种商业微系统Enterotube和Minitek进行了比较研究,以便鉴定肠杆菌科细菌,并与我们实验室常用的宏观方法组合所构成的参考系统进行比较。我们检查了401株革兰氏阴性杆菌的细菌培养物,这些培养物来自我们收到用于细菌学诊断的临床材料(粪便、尿液、咽拭子、阴道拭子、尿道拭子和痰液),我们认为它们属于肠杆菌属。401份培养物中有390份显示为肠杆菌属。它们给出的生化反应表明,Enterotube和Minitek与常规系统在以下测试中具有良好的一致性:葡萄糖(酸和气体)、赖氨酸和鸟氨酸脱羧酶、H2S和吲哚的产生、苯丙氨酸脱氨酶和脲酶;而在乳糖发酵和柠檬酸盐利用方面,我们存在一些具有统计学意义的不一致。在山梨醇发酵方面,我们也有显著的E/C不一致,而Minitek的结果是可接受的。这些记录建议在专业细菌学实验室中使用传统测试。