Bahabayi Ayibaota, Xiong Ziqi, Li Qi, Wang Guochong, Liu Ruiqing, Zhang Ke, Zhang Zhonghui, Gao Yiming, Wang Pingzhang, Liu Chen
Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
Department of Immunology, NHC Key Laboratory of Medical Immunology (Peking University), Medicine Innovation Center for Fundamental Research on Major Immunology-related Diseases, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China; Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing, China.
Int Immunopharmacol. 2025 Jan 3;145:113822. doi: 10.1016/j.intimp.2024.113822. Epub 2024 Dec 10.
This study aimed to investigate the role of CD55 in regulatory T cells (Tregs) and clarify its clinical relevance in rheumatoid arthritis (RA).
Flow cytometry was used to examine the expression of Helios and CTLA-4 in CD55 + and CD55- Tregs in mouse peripheral blood and spleen. FoxP3 mice were employed to analyze the in vitro inhibitory function of CD55 + and CD55-Tregs. We compared CD55 expression and function in control and CIA mice. Additionally, the expression of Helios, PD-1, and TIGIT in CD55 + Tregs was examined in healthy controls and RA patients. Correlation analysis and receiver operating characteristic (ROC) curves were used to assess the clinical utility of CD55-related T cell subgroups in RA diagnosis.
High CD55 expression was observed in CD4 + T cells and Tregs, with significantly higher levels in peripheral blood than in the spleen in mice. CD55-Tregs exhibited increased expression of Helios and CTLA-4, and a more pronounced suppressive function compared to CD55 + Tregs in mice. Additionally, CD55 expression in Tregs from peripheral blood and spleen of CIA mice was significantly reduced. In humans, peripheral blood CD55- Tregs expressed higher levels of Helios and TIGIT. Moreover, CD55 + Tregs were markedly reduced in RA patients and correlated with clinical indicators of RA, demonstrating potential as diagnostic markers.
CD55 + Tregs represent a subset of Tregs with weaker functionality and were decreased in RA and could assist the diagnosis of RA.
本研究旨在探讨CD55在调节性T细胞（Tregs）中的作用，并阐明其在类风湿关节炎（RA）中的临床相关性。
采用流式细胞术检测小鼠外周血和脾脏中CD55 +和CD55 - Tregs中Helios和CTLA-4的表达。利用FoxP3小鼠分析CD55 +和CD55 - Tregs的体外抑制功能。我们比较了对照小鼠和胶原诱导性关节炎（CIA）小鼠中CD55的表达和功能。此外，检测了健康对照者和RA患者中CD55 + Tregs中Helios、PD-1和TIGIT的表达。采用相关性分析和受试者工作特征（ROC）曲线评估CD55相关T细胞亚群在RA诊断中的临床应用价值。
在CD4 + T细胞和Tregs中观察到高CD55表达，小鼠外周血中的水平显著高于脾脏。与CD55 + Tregs相比，CD55 - Tregs在小鼠中表现出Helios和CTLA-4表达增加以及更明显的抑制功能。此外，CIA小鼠外周血和脾脏中Tregs的CD55表达显著降低。在人类中，外周血CD55 - Tregs表达更高水平的Helios和TIGIT。此外，RA患者中CD55 + Tregs明显减少，且与RA的临床指标相关，显示出作为诊断标志物的潜力。
CD55 + Tregs代表功能较弱的Tregs亚群，在RA中减少，可辅助RA的诊断。
