病毒nsP2 ATP酶抑制剂的鉴定
Identification of Inhibitors of virus nsP2 ATPase.
作者信息
Navarro Hernan, Scott John E, Smith Ginger R, Ghiabi Pegah, Gibson Elisa, Loppnau Peter, Harding Rachel J, Hossain Mohammad Anwar, Bose Muthu Ramalingam, Pearce Kenneth H, Merten Eric M, Willson Timothy M, Brown Peter J
机构信息
Department of Pharmaceutical Sciences, Biomanufacturing Research Institute and Technology Enterprise (BRITE), North Carolina Central University, Durham, NC 27707, USA.
READDI AViDD Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
出版信息
bioRxiv. 2025 Jan 14:2024.12.02.625520. doi: 10.1101/2024.12.02.625520.
Abstract
Non-structural protein 2 (nsP2), which plays an essential role in replication of CHIKV, contains a protease, helicase, and methyltransferase-like domain. We executed a simple a screen using malachite green to detect compounds that decreased ATP hydrolysis and tested a library of diverse compounds to find inhibitors of CHIKV nsP2 helicase.
摘要
非结构蛋白2（nsP2）在基孔肯雅病毒（CHIKV）复制中起关键作用，它包含一个蛋白酶、解旋酶和类甲基转移酶结构域。我们进行了一项简单的孔雀石绿筛选，以检测能降低ATP水解的化合物，并测试了一个多样化化合物库，以寻找CHIKV nsP2解旋酶的抑制剂。
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