Ruggeri Elena, Klohonatz Kristin, Durrant Barbara, Sirard Marc-André
Reproductive Sciences, Conservation Science Wildlife Health, San Diego Zoo Wildlife Alliance, Escondido, CA 92027, USA.
Center for Research on Reproduction and Women's Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Animals (Basel). 2024 Dec 7;14(23):3538. doi: 10.3390/ani14233538.
In recent years, biomarkers in granulosa cells (GC) have been determined and associated in several species with oocyte maturation, in vitro fertilization success, and embryo development outcomes. The identification of biomarkers of oocyte competence can aid in improving assisted reproductive technologies (ARTs) in the southern white rhino (SWR). This study aimed to identify biomarkers present in SWR GC associated with oocytes that either did or did not mature in vitro. We evaluated follicle development (FD), meiotic competence (MC), cell death and atresia (CDA), and embryonic genome activation (EGA). Our objective was to design biomarkers to predict oocyte in vitro maturation results in the SWR. RNA was isolated from GC obtained during ovum pick up (OPU) for qPCR analysis. Overall, 22 genes were assessed, and nine were differentially expressed between GC from oocytes that did or did not mature in vitro (FD-GDF9 and mTOR; MC-GGPS1, JMY, and NPR2; CDA-COL4A1, MACIR, and TMPO; EGA-NFYA). From these data, we determined that GC can be used as a predictor for oocyte in vitro maturation outcome in the SWR. Our results provide crucial information needed to improve in vitro maturation and ARTs in this species.
近年来，颗粒细胞（GC）中的生物标志物已在多个物种中得到测定，并与卵母细胞成熟、体外受精成功率和胚胎发育结果相关联。鉴定卵母细胞能力的生物标志物有助于改进南方白犀牛（SWR）的辅助生殖技术（ARTs）。本研究旨在鉴定SWR GC中与体外成熟或未成熟的卵母细胞相关的生物标志物。我们评估了卵泡发育（FD）、减数分裂能力（MC）、细胞死亡和闭锁（CDA）以及胚胎基因组激活（EGA）。我们的目标是设计生物标志物来预测SWR中卵母细胞的体外成熟结果。从取卵（OPU）过程中获得的GC中分离RNA用于qPCR分析。总共评估了22个基因，其中9个基因在体外成熟或未成熟的卵母细胞的GC之间存在差异表达（FD - GDF9和mTOR；MC - GGPS1、JMY和NPR2；CDA - COL4A1、MACIR和TMPO；EGA - NFYA）。根据这些数据，我们确定GC可用作SWR中卵母细胞体外成熟结果的预测指标。我们的结果为改善该物种的体外成熟和ARTs提供了所需的关键信息。
