Bussan Derek D, Nielsen Forrest H, Douvris Chris, Kelzenberg Brett, Grimestad Allison, Cao Jay J
United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Grand Forks Human Nutrition Research Center, Grand Forks, ND 58203, USA.
Theobald Science Center, Department of Biological and Chemical Sciences, New York Institute of Technology, Old Westbury, NY 11568, USA.
Molecules. 2024 Nov 22;29(23):5517. doi: 10.3390/molecules29235517.
An environmentally compatible and less costly (greener) analytical method for the digestion of bone meal samples using microwave-assisted dilute nitric acid (HNO) was developed and optimized. The method, employing a mixture of 1 mL concentrated HNO and 4 mL of deionized water, offered a comparable performance to the conventional method using 5 mL of concentrated HNO. The accuracy of the method was validated by using certified reference material NIST 1486 (Bone Meal); percentage recoveries were within ±15% for all eight certified elements. Statistical analysis revealed no significant differences ( > 0.05) in percentage recoveries between the green and conventional methods for all elements except calcium. The greenness of the developed method was evaluated by using the analytical Eco-Scale, achieving a score of 87, categorizing it as an "excellent green analysis" method. This research highlights the potential for adopting greener practices in trace element analysis that reduce the environmental impact and safety risks associated with concentrated acids.
开发并优化了一种环境友好且成本较低（更绿色）的分析方法，该方法使用微波辅助稀硝酸（HNO₃）消解骨粉样品。该方法采用1 mL浓硝酸和4 mL去离子水的混合物，其性能与使用5 mL浓硝酸的传统方法相当。通过使用认证参考物质NIST 1486（骨粉）验证了该方法的准确性；所有八种认证元素的回收率在±15%以内。统计分析表明，除钙元素外，绿色方法和传统方法对所有元素的回收率均无显著差异（p>0.05）。使用分析生态量表评估了所开发方法的绿色程度，得分为87分，将其归类为“优秀的绿色分析”方法。本研究突出了在微量元素分析中采用更绿色做法的潜力，这些做法可减少与浓酸相关的环境影响和安全风险。
