Musapoor Saeed, Davoodian Najmeh, Kadivar Ali, Ahmadi Ebrahim, Nazari Hassan
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Vet Med Sci. 2025 Jan;11(1):e70134. doi: 10.1002/vms3.70134.
The process of maturing ovine oocyte in vitro has not yet been raised with acceptable results.
This study was designed to evaluate the γ-oryzanol effect as a supplement of maturation media on the development of ovine oocytes to blastocyst.
Aspirated from ovine ovaries, morphologically normal cumulus-oocyte complexes (COCs) were matured in media supplemented with or without 5 µM γ-oryzanol. Matured oocytes were divided into two parts: one evaluated for their nuclear maturation, the level of GSH and ROS, mitochondrial membrane potential (MMP) and the pattern of transcription in oocytes and respective cumulus cells (CCs), and another subjected to fertilisation and culture to assess the development of oocytes to the blastocyst.
γ-Oryzanol improved the proportion of cleaved embryos and total blastocysts in the treated group, which was linked to improved MMP, higher levels of intracellular GSH and lower levels of ROS. A lower proportion of MI and GVBD was recorded for treated oocytes in comparison with control, although the proportion of MII oocytes was not different between groups. The treated oocytes and CCs showed downregulation of genes related to apoptosis (BAX and CASP-9) and upregulation of genes related to antioxidative status (NRF2, CAT and SOD). In conclusion, our results demonstrated the improved developmental outcome of supplemented oocytes so that the antioxidant response and higher enzymatic activity were maintained, and the generation of ROS was turned off; therefore, a novel alternative for counteracting oxidative stress in ovine oocytes undergoing maturation was offered by γ-oryzanol through an antioxidative pathway.
绵羊卵母细胞的体外成熟过程尚未取得令人满意的结果。
本研究旨在评估γ-谷维素作为成熟培养基补充剂对绵羊卵母细胞发育至囊胚的影响。
从绵羊卵巢中吸出形态正常的卵丘-卵母细胞复合体（COCs），在添加或不添加5 μM γ-谷维素的培养基中进行成熟培养。成熟的卵母细胞分为两部分：一部分评估其核成熟度、谷胱甘肽（GSH）和活性氧（ROS）水平、线粒体膜电位（MMP）以及卵母细胞和相应卵丘细胞（CCs）中的转录模式；另一部分进行受精和培养，以评估卵母细胞发育至囊胚的情况。
γ-谷维素提高了处理组中分裂胚和总囊胚的比例，这与改善的MMP、更高水平的细胞内GSH和更低水平的ROS有关。与对照组相比，处理组卵母细胞的MI和生发泡破裂（GVBD）比例较低，尽管两组间MII期卵母细胞的比例没有差异。处理后的卵母细胞和CCs显示出与细胞凋亡相关基因（BAX和CASP-9）的下调以及与抗氧化状态相关基因（NRF2、CAT和SOD）的上调。总之，我们的结果表明，补充γ-谷维素的卵母细胞发育结果得到改善，从而维持了抗氧化反应和更高的酶活性，并抑制了ROS的产生；因此，γ-谷维素通过抗氧化途径为应对成熟过程中的绵羊卵母细胞氧化应激提供了一种新的选择。
