Keller Jonathan Michel, Klamminger Gilbert Georg, Tschernig Thomas, Linxweiler Barbara, Korac Leida, Wagner Mathias, Solomayer Erich Franz, Kasoha Mariz
Institute of Anatomy and Cell Biology, Saarland University, Campus Homburg, Homburg, Germany; Saarland University Medical Center (UKS), Department of Gynecology and Obstetrics, Homburg 66424, Germany.
Saarland University (USAAR) and Saarland University Medical Center (UKS), Department of General and Special Pathology, Homburg 66424, Germany; Department of Obstetrics and Gynecology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz 55131, Germany.
Ann Anat. 2025 Feb;258:152371. doi: 10.1016/j.aanat.2024.152371. Epub 2024 Dec 15.
Although to date the pathogenesis of endometriosis remains largely unexplained, it is known that processes of migration, proliferation and revascularization and thus calcium as a messenger substance play an important role. Consecutively, the present study examines the immunohistochemical expression of the calcium transient receptor potential channels 3 and 6 (TRPC3 and TRPC6) in ectopically located (outside the uterine cavity) endometrial tissue.
Laparoscopically collected and histomorphologically verified endometriosis tissues from several different intraabdominal locations were examined (n = 20) and immunohistochemical stainings were performed with anti-TRPC3 and anti-TRPC6 antibodies (Alomone Labs, Jerusalem). Hereby, eutopic endometrium served as a healthy control cohort (n = 6). Staining patterns were evaluated using a modified immunoreactive score (IRS) and exploratory statistical analysis was performed, aiming to determine relations of staining intensities with associated clinical parameters such as rASRM stage and location.
We determined a strong cytoplasmatic TRPC3 and TRPC6 expression in all ectopic endometrial glandular formations, albeit with focally varying staining intensities. Within our cohort, we did not verify a statistically significant difference of TRPC3 and TRPC6 expression between endometriosis patients and a healthy control group or between different clinical affections (rASRM stages).
Our study confirms - to our knowledge for the first time - the successful immunohistochemical assessment of TRPC3 and TRPC6 in endometriosis, setting the basis for future studies aiming at evaluating not only clinical aspects of TRPC3 and TRPC6 expression but also shedding light on its function in the pathophysiological context of endometriosis.
尽管迄今为止子宫内膜异位症的发病机制在很大程度上仍未得到解释，但已知迁移、增殖和血管再生过程以及钙作为一种信使物质发挥着重要作用。因此，本研究检测了异位（子宫腔外）子宫内膜组织中瞬时受体电位钙通道3和6（TRPC3和TRPC6）的免疫组化表达。
检查了通过腹腔镜收集并经组织形态学验证的来自几个不同腹腔内位置的子宫内膜异位症组织（n = 20），并用抗TRPC3和抗TRPC6抗体（Alomone Labs，耶路撒冷）进行免疫组化染色。在此，在位子宫内膜作为健康对照队列（n = 6）。使用改良的免疫反应评分（IRS）评估染色模式，并进行探索性统计分析，旨在确定染色强度与相关临床参数（如rASRM分期和位置）之间的关系。
我们确定在所有异位子宫内膜腺管结构中均有强烈的细胞质TRPC3和TRPC6表达，尽管染色强度存在局部差异。在我们的队列中，我们未证实子宫内膜异位症患者与健康对照组之间或不同临床病变（rASRM分期）之间TRPC3和TRPC6表达存在统计学显著差异。
据我们所知，我们的研究首次证实了在子宫内膜异位症中成功进行TRPC3和TRPC6的免疫组化评估，为未来的研究奠定了基础，这些研究不仅旨在评估TRPC3和TRPC6表达的临床方面，还旨在阐明其在内膜异位症病理生理背景下的功能。
