Zhang Cui, Nie Yugang, Li Jian, Ji Xiaoyu, Han Mengjie, Qin Rong, Liu Yuqiu, Xing Wenge, Qiu Maofeng, Li Ning, Liu Zhongfu
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Institute of Sexually Transmitted Diseases and AIDS Prevention and Treatment, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China.
J Virol Methods. 2025 Apr;333:115102. doi: 10.1016/j.jviromet.2024.115102. Epub 2024 Dec 16.
Hepatitis C virus (HCV) resistance-associated substitutions (RASs) have a significant impact on the treatment of HCV with direct-acting antivirals (DAAs). However, limited research has been conducted, and no standardized methods for detecting RASs in mainland China.
To develop and apply a novel method for detecting HCV RASs in HCV RNA-positive patients in Linzhou, China.
In total, 103 HCV RNA-positive serum specimens and epidemiological questionnaires were collected. A PCR method for detecting HCV RASs encompassing the NS3 to NS5B region was developed.
Demographic analysis revealed a predominance of females (66/103, 64.1 %), with an average age of 70 years. Genotype 1b (GT1b) (17/103, 16.5 %) and GT2a (86/103, 83.5 %) were identified. The prevalence of RASs was higher (17/17, 100 %) in GT1b than in GT2a (7/86, 8 %). In GT1b, a higher frequency of RASs was observed in the NS5B region (17/17, 100 %) than in the NS3 (14/17, 82 %) and NS5A (10/17, 59 %) regions. C316N was the most prevalent, followed by S122G (71 %) and R30Q (35 %).
We introduced an innovative approach for the detection of HCV RASs and provided a wealth of information on HCV RASs in Linzhou, China. The findings support the cautious selection of treatment regimens, potentially improving patient outcomes.
丙型肝炎病毒（HCV）耐药相关替代位点（RASs）对使用直接抗病毒药物（DAAs）治疗HCV有重大影响。然而，相关研究有限，中国大陆尚无检测RASs的标准化方法。
在中国林州开发并应用一种检测HCV RNA阳性患者中HCV RASs的新方法。
共收集103份HCV RNA阳性血清标本和流行病学调查问卷。开发了一种检测涵盖NS3至NS5B区域的HCV RASs的PCR方法。
人口统计学分析显示女性占多数（66/103，64.1%），平均年龄为70岁。鉴定出1b型（GT1b）（17/103，16.5%）和2a型（GT2a）（86/103，83.5%）。GT1b中RASs的发生率（17/17，100%）高于GT2a（7/86，8%）。在GT1b中，NS5B区域RASs的发生率（17/17，100%）高于NS3（14/17，82%）和NS5A（10/17，59%）区域。C316N最为常见，其次是S122G（71%）和R30Q（35%）。
我们引入了一种创新方法来检测HCV RASs，并提供了中国林州HCV RASs的丰富信息。这些发现支持谨慎选择治疗方案，可能改善患者预后。
