Silverman D G, Norton K J, Brousseau D A
Surgery. 1985 Feb;97(2):185-93.
Since its introduction in 1980, fiberoptic fluorometry has improved the precision and objectivity of the fluorescein test as a means to delineate nutritive blood flow and predict tissue viability. This study was undertaken primarily to determine if fiberoptic fluorometry could be repeated reliably at close time intervals. Four injections of fluorescein dye were administered at varying dose and time schedules to determine what factors influenced the repeatability of this technique. The results confirm the high accuracy of fluorometric indices as a means to predict the ultimate fate of a skin flap. A method of scanning the flap within 2 minutes after injection of 1.5 mg/kg of fluorescein dye correctly predicted the fate of almost 100% of flap sections. Repeated injections of 1.5, 3.0, and 6.0 mg/kg of dye at 15-minute intervals provided virtually identical predictions of flap viability. Thus fluorometry is readily repeatable and should be of value in assessing the state of tissue perfusion as well as changes in perfusion caused by pharmacologic manipulation or surgical intervention.
自1980年引入以来，光纤荧光测定法提高了荧光素测试的精度和客观性，作为描绘营养性血流和预测组织活力的一种手段。本研究主要旨在确定光纤荧光测定法能否在较短时间间隔内可靠地重复进行。以不同剂量和时间安排注射四次荧光素染料，以确定哪些因素影响该技术的可重复性。结果证实了荧光测定指标作为预测皮瓣最终命运手段的高准确性。在注射1.5mg/kg荧光素染料后2分钟内扫描皮瓣的方法正确预测了几乎100%皮瓣切片的命运。每隔15分钟重复注射1.5、3.0和6.0mg/kg染料对皮瓣活力的预测几乎相同。因此，荧光测定法易于重复，在评估组织灌注状态以及药物操作或手术干预引起的灌注变化方面应具有价值。
