Liang Qiaolan, Wei Liexin, Chen Ying'e, Xu Bingliang, Zhang Na
College of Plant Protection, Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, Lanzhou, China.
Front Microbiol. 2024 Dec 3;15:1489999. doi: 10.3389/fmicb.2024.1489999. eCollection 2024.
Lily bulb rot disease has harmed edible lily in recent years, resulting in yield losses in China. As a results, both morphological and molecular techniques must be used to confirm the etiology of storage bulb rot disease on lily bulbs. Lily bulbs with indications of rot symptoms during storage were gathered in Lanzhou, Gansu Province, China. A strain was isolated and its morphologically characterized as a specie. Pathogenicity tests further confirmed that the strain caused apparent symptoms on lily bulbs which were consistent with those seen in the field. The pathogenicity of to lily bulb was 100%, and morphological identification showed that the mycelia of the pathogen were white and villous, with septate, branched, and acicular bodies and obvious lock-shaped joints. The mycelia had uneven thickness, ranging from 1.03 to 3.06 μm, and turned gray-white at the later growth stage. Moreover, the pathogen formed a mycelial column on potato dextrose agar (PDA) medium, which formed a coral-like fruiting body primordium, which split, forming pileus for spore production. The spores were colorless and cylindrical, with an oblique tip and a size of 4.6-7.2 μm × 2.2-2.7 μm. The isolate was deduced as based on phylogenetic analysis with 2 genes (ribosomal DNA-ITS and LSU) as well as morphological characteristics and cultural features, the isolate was identified . Soluble starch, yeast extract, temperature of 30°C, pH 7, relative humidity of 100%, and complete dark were shown to be the optimum culture conditions for surface mycelium growth. In conclusion, this is the first report of causing bulb rot of edible lilies in China. The study provides a basis for more effective control strategies for lily bulb rot disease.
近年来，百合鳞茎腐烂病对食用百合造成了危害，导致中国百合产量损失。因此，必须同时运用形态学和分子技术来确定百合种球贮藏期腐烂病的病因。在中国甘肃省兰州市收集了贮藏期间出现腐烂症状的百合种球。分离出一个菌株，并对其进行形态学鉴定，确定为一个物种。致病性测试进一步证实，该菌株在百合种球上引起的明显症状与田间观察到的症状一致。该菌株对百合种球的致病性为100%，形态学鉴定表明，病原菌的菌丝为白色、绒毛状，有隔膜、分枝，呈针状，有明显的锁状联合。菌丝粗细不均，直径在1.03至3.06μm之间，生长后期变为灰白色。此外，病原菌在马铃薯葡萄糖琼脂（PDA）培养基上形成菌丝柱，进而形成珊瑚状子实体原基，原基分裂形成菌盖以产生孢子。孢子无色、圆柱形，顶端倾斜，大小为4.6 - 7.2μm×2.2 - 2.7μm。基于对2个基因（核糖体DNA-ITS和LSU）的系统发育分析以及形态特征和培养特性，推断该分离株为[具体物种名称未给出]。结果表明，可溶性淀粉、酵母提取物、30°C温度、pH值7、100%相对湿度和完全黑暗是表面菌丝生长的最佳培养条件。总之，这是中国首次报道[具体病原菌未明确给出]引起食用百合鳞茎腐烂。该研究为更有效的百合鳞茎腐烂病防治策略提供了依据。
