Currey Laura, Harris Lachlan, Piper Michael
School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia.
BMC Res Notes. 2024 Dec 24;17(1):382. doi: 10.1186/s13104-024-07008-y.
The Polycomb Repressive Complex 2 (PRC2) regulates neural stem cell behaviour during development of the cerebral cortex, yet how the loss of PRC2 developmentally influences cell identity in the mature brain is poorly defined. Using a mouse model in which the PRC2 gene Embryonic ectoderm development (Eed) was conditionally deleted from the developing mouse dorsal telencephalon, we performed single nuclei RNA sequencing (snRNA-seq) on the cortical plate of an adult heterozygote Eed knockout mouse and an adult homozygote Eed knockout mouse compared to a littermate control. This work was part of a larger effort to understand consequences of mutations to PRC2 within the mature brain.
Here we provide snRNA-seq data from the cortical plate of an adult heterozygous conditional Eed knockout, an adult homozygous conditional Eed knockout and an adult control mouse. This data provides insight on how loss of PRC2 function during development affects cell identity in the mature cortex.
多梳抑制复合物2（PRC2）在大脑皮质发育过程中调节神经干细胞行为，但PRC2的缺失在发育过程中如何影响成熟大脑中的细胞身份仍不清楚。我们使用一种小鼠模型，其中PRC2基因胚胎外胚层发育（Eed）在发育中的小鼠背侧端脑中被条件性删除，与同窝对照相比，我们对成年杂合子Eed敲除小鼠和成年纯合子Eed敲除小鼠的皮质板进行了单核RNA测序（snRNA-seq）。这项工作是了解成熟大脑中PRC2突变后果的更大努力的一部分。
在这里，我们提供了来自成年杂合子条件性Eed敲除、成年纯合子条件性Eed敲除和成年对照小鼠皮质板的snRNA-seq数据。该数据提供了关于发育过程中PRC2功能丧失如何影响成熟皮质中细胞身份的见解。
