Putta Chandra Lekha, Buddhiraju Hima Sree, Yadav Dokkari Nagalaxmi, Basa Apoorva, Rengan Aravind Kumar
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Hyderabad 502285, India.
ACS Appl Bio Mater. 2025 Jan 20;8(1):661-675. doi: 10.1021/acsabm.4c01533. Epub 2024 Dec 24.
Triple-negative breast cancer (TNBC) is known for its aggressive nature, typically presenting as high-grade tumors that grow and spread quickly in all breast cancer types. Several studies have reported a strong correlation between cancer and microbial infections due to a compromised immune system. The most frequent infection associated with surface malignancies, including breast cancer, is Candidiasis, which is majorly caused by . This study reports the development and characterization of the drug Baicalein (B) and NIR dye IR780 (IR) coloaded liposomes (BIRLs) as a multifunctional nanoplatform for treating fungal infections and TNBC. BIRLs were prepared by using hydrogenated soybean phosphatidylcholine as the lipid matrix, enhancing both the drug and dye solubility and therapeutic efficacy. The synthesized BIRLs-mediated photothermal therapy (PTT) exhibited significant synergistic antifungal efficacy when tested against . The biocompatibility of BIRLs was studied in fibroblast cell lines and zebrafish embryos. BIRLs demonstrated promising photothermal and photodynamic effects, synergistically enhancing tumor ablation and reactive oxygen species (ROS) generation upon near-infrared (NIR) laser irradiation. In vitro studies revealed that BIRLs exhibit potent anticancer activity in two-dimensional (2D) cell cultures and three-dimensional (3D) tumor spheroids, significantly inhibiting cancer cell proliferation and migration. The dual therapeutic effect of BIRLs was additionally demonstrated by their ability to inhibit fungal growth, addressing common complications in cancer patients with compromised immune systems. Overall, the results highlighted the promising application of BIRLs as a versatile nanoplatform for synergistic cancer therapy and as an antifungal agent, with the potential to significantly improve outcomes for TNBC patients.
三阴性乳腺癌（TNBC）以其侵袭性而闻名，通常表现为高级别肿瘤，在所有乳腺癌类型中生长和扩散迅速。几项研究报告称，由于免疫系统受损，癌症与微生物感染之间存在密切关联。与包括乳腺癌在内的体表恶性肿瘤相关的最常见感染是念珠菌病，主要由……引起。本研究报告了载有药物黄芩素（B）和近红外染料IR780（IR）的脂质体（BIRLs）的开发和特性，作为一种用于治疗真菌感染和TNBC的多功能纳米平台。通过使用氢化大豆磷脂酰胆碱作为脂质基质制备BIRLs，提高了药物和染料的溶解度以及治疗效果。合成的BIRLs介导的光热疗法（PTT）在针对……进行测试时表现出显著的协同抗真菌功效。在成纤维细胞系和斑马鱼胚胎中研究了BIRLs的生物相容性。BIRLs表现出有前景的光热和光动力效应，在近红外（NIR）激光照射下协同增强肿瘤消融和活性氧（ROS）生成。体外研究表明，BIRLs在二维（2D）细胞培养和三维（3D）肿瘤球体中表现出强大的抗癌活性，显著抑制癌细胞增殖和迁移。BIRLs抑制真菌生长的能力进一步证明了其双重治疗效果，解决了免疫系统受损的癌症患者的常见并发症。总体而言，结果突出了BIRLs作为一种用于协同癌症治疗的多功能纳米平台和抗真菌剂的有前景的应用，有可能显著改善TNBC患者的治疗结果。
