Dehghan Ali Asghar, Ghaderi Reza, Karegar Akbar, Hesar Abbas Mokaram
Department of Plant Protection, School of Agriculture, Shiraz University, 71441-65186, Shiraz, Iran.
Department of Plant Protection, Faculty of Agriculture, Afagh Higher Education Institute, Urmia 5756151818, Iran.
J Nematol. 2024 Dec 24;56(1):20240048. doi: 10.2478/jofnem-2024-0048. eCollection 2024 Mar.
In this survey, 14 populations of were collected from the rhizosphere of eight fruit and nut trees in Fars province, Southern Iran. The phylogenetic relationships of these populations with other representatives of the species were investigated using sequences of cytochrome c oxidase subunit 1 mitochondrial gene () and D2-D3 expansion fragments of 28S rDNA. Phylogenetic studies indicated a close relationship of the currently sequenced populations with known haplotype groups (HG) in the tree and revealed two separate lineages in the 28S rDNA tree. Moreover, the genetic diversity of the populations was analyzed using seven ISSR primers as molecular markers. The estimated genetic diversity among populations regarding associated trees and geographic regions were low values of 3.3% and 5.9%, respectively, indicating high gene flow among the recovered nematode populations. On the other hand, the estimated fixation index (F) was higher for associated plants than for geographic regions (0.611 0.504) indicating that plant-based population segregation better explains genetic diversity in this species. This work expands our knowledge of the genetic structure of this cosmopolitan species of plant-parasitic nematodes.
在本次调查中，从伊朗南部法尔斯省的8种果树和坚果树的根际收集了14个群体。利用细胞色素c氧化酶亚基1线粒体基因（）序列和28S rDNA的D2-D3扩增片段，研究了这些群体与该物种其他代表群体之间的系统发育关系。系统发育研究表明，目前测序的群体与树中已知单倍型组（HG）关系密切，并在28S rDNA树中揭示了两个独立的谱系。此外，使用7种ISSR引物作为分子标记分析了群体的遗传多样性。关于相关树木和地理区域的群体间估计遗传多样性分别为较低的值3.3%和5.9%，表明回收的线虫群体间基因流较高。另一方面，相关植物的估计固定指数（F）高于地理区域（0.611对0.504），表明基于植物的群体隔离能更好地解释该物种的遗传多样性。这项工作扩展了我们对这种世界性植物寄生线虫遗传结构的认识。
